Efficacies of PGF2α, Ovsynch, and CIDR Protocols on Synchronization of Estrus in Buffaloes: A Comparative Study

Biman Chandra  Roy; Md. Sayaduzzaman  Arafath; Md Hasanur  Alam; Jannatul  Bari; Gautam Kumer  Deb; Nasrin Sultana  Juyena; Mohammad  Moniruzzaman

doi:10.6000/1927-520X.2025.14.15

Authors

Biman Chandra Roy Department of Animal Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
Md. Sayaduzzaman Arafath Department of Animal Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
Md Hasanur Alam Department of Animal Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
Jannatul Bari Department of Animal Products Technology, Patuakhali Science and Technology University, Babuganj, Barisal, Bangladesh
Gautam Kumer Deb Biotechnology Division, Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh
Nasrin Sultana Juyena Department of Surgery and Obstetrics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
Mohammad Moniruzzaman Department of Animal Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

DOI:

https://doi.org/10.6000/1927-520X.2025.14.15

Keywords:

Calving, CIDR, conception, estrus synchronization, GnRH, PGF2α

Abstract

The present study aimed to compare the efficacies of PGF_2α,GnRH, and CIDR on estrus synchronization of native buffaloes in Bangladesh. A total of 93 buffaloes of second to fifth parity were treated either with PGF_2α, Ovsynch, or CIDR protocol. In the PGF_2α experiment, buffaloes were treated with either 500 μg (n=20) or 875 μg (n=20) of cloprostenol, and artificial inseminations (AI) were done at 72 hrs after PGF_2α injection. In the Ovsynch protocol, buffaloes were treated with 200 μg (n=11) or 350 µg (n=16) of GnRH at day 0, followed by 875 µg of PGF_2α at day 7, and again 200 μg or 350 µg of GnRH at day 9, and AI was performed at 16 hrs after the second GnRH administration. In the CIDR protocol, a CIDR implant was placed intravaginally for 12 days with either 500 μg (n=13) or 875 μg (n=13) of cloprostenol at 24 hrs before the removal of the CIDR, and AI was performed at 72 hrs after the removal of the CIDR. The results showed that estrus response and conception rates did not differ significantly between PGF_2α and GnRH protocols. Higher doses of PGF_2α and GnRH did not result in any significant increase in estrus response in buffaloes. CIDR induced estrus in all buffaloes in both doses of PGF_2α. Estrus rate was significantly higher (P=0.035) in buffaloes of the CIDR protocol than in the PGF_2α and Ovsynch groups. Conception rates of buffaloes did not differ significantly (P=0.823) among the protocols. The calving rates were higher (P=0.278) in buffaloes synchronized with CIDR than in PGF_2α and Ovsynch groups. The costs of materials per buffalo synchronized, conceived, and calved were higher in the CIDR protocol and lower in the PGF_2α than in other protocols. However, considering all the expenses and calving rates, costs per buffalo calves were cheaper in the CIDR group with a higher dose of PGF_2α. In conclusion, CIDR can be applied to increase the reproductive efficiency of buffaloes.

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