Innovative Use of Endogenous Enzymes to Enhance Silage Chemical Quality of Corn Straw for Buffalo (Bubalusbubalis) Feed in Samosir Island

Authors

  • Nurzainah Ginting Animal Production Program Study, Faculty of Agriculture, Universitas Sumatera Utara, Medan, Indonesia and Head of Eco Enzyme Team of UI Greenmetric World University Ranking Network, Indonesia
  • Raden Edhy Mirwandhono Animal Production Program Study, Faculty of Agriculture, Universitas Sumatera Utara, Medan, Indonesia
  • Muhammad Adanan Purba Animal Production Program Study, Faculty of Agriculture, Universitas Sumatera Utara, Medan, Indonesia
  • Muhammad Amran Nasution Animal Production Program Study, Faculty of Agriculture, Universitas Sumatera Utara, Medan, Indonesia
  • Winda Fransisca Saragih Animal Production Program Study, Faculty of Agriculture, Universitas Sumatera Utara, Medan, Indonesia
  • Nurjama’yah br. Ketaren Animal Production Program Study, Faculty of Agriculture, Universitas Sumatera Utara, Medan, Indonesia
  • Anjas Asmara Samsudin Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, Selangor Dahrul Ehsan, Malaysia

DOI:

https://doi.org/10.6000/1927-520X.2025.14.16

Keywords:

ADF, Dry season, enzymes, fermented corn straw, NDF, rumen

Abstract

On Samosir Island, there is an annual shortage of buffalo feed during the dry season. However, this feed shortage has caused farmers to scramble to find substitute feed. Still, it has also caused stress in the buffalo, which has led to the emergence of Haemorrhagic septicemia (HS), as Samosir is an endemic area (HS). Beginning in 2024, corn cultivation began to be widely practiced on the island of Samosir, which generates a significant amount of waste such as corn straw. This research focuses on preparing feed for buffalo (Bubalus bubalis) using fermented corn straw, with the fermentation process utilizing endogenous enzymes derived from rumen fermentation (EERF). It was obtained from fermented 100- day buffalo rumen, where the buffalo rumen comes from buffalo that consume corn straw. This study used a completely randomized design, a 3×3factorial with three replications. Factor I was various doses of EERF(2%, 4%,6%),and Factor II was different fermentation times(5d,10d, 15d).Parameters that were observed in this study were chemical quality: Dry Matter (DM), Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), Crude Protein (CP), and pH. Previously, isolation on EERF was carried out to identify the dominant fiber-degrading colonies, as they produce enzymes. Analysis of the potential of corn straw for buffalo feed was conducted by calculating corn straw production from the corn harvest area on Samosir Island. The result of this study is that corn straw fermentation using endogenous enzymes improves the chemical quality of silage, such as DM increasing from 38.59 to 46.17, NDF from 46.23 to 40.48, ADF from 30.53 to 24.21, CP from 9.25 to 9.86, and pH from 5.15 to 4.76. Through this improvement in nutritional quality, the dietary needs of buffaloes are met. This corn straw is sufficient for 9,565,101 buffaloes. Since corn cultivation has become intensive, there is a tendency of HS cases to decrease, with only 6 instances of HS in 2024 and 13 cases up to June 2025, while in 2023 there were 202 cases.

References

Ginting N, Hasnudi H, Mirwandhono E, Hellyward J, Sulistyowati E, Hafid H, et al. A Study of Infection Areas and Social Assessment Findings in Hs (haemorrhagic Septicaemia)-Affected Buffaloes on Samosir Island, Indonesia. EEC J 2022; 28: 15-23. DOI: https://doi.org/10.53550/EEC.2022.v28i06s.003

Prihandini PW, Tribudi YA, Hariyono DNH, Sari APZNL, Praharani L, Handiwirawan E, et al. Biodiversity of Indonesian Indigenous Buffalo: First Review of the Status, Challenges, and Development Opportunities. Veterinary World 2023; 16(5): 2217. DOI: https://doi.org/10.14202/vetworld.2023.2217-2229

El Debaky HA, Kutchy NA, Ul-Husna A, Indriastuti R, Akhter S, Purwantara B, et al. Potential of Water Buffalo in World Agriculture: Challenges and Opportunities. Applied Animal Science 2019; 35(2): 255-268. DOI: https://doi.org/10.15232/aas.2018-01810

Rohaeni ES, Santoso AD, Ariningsih E, Widaningsih N, Hutahaean L, Priyanto D, et al. Analysing the Sustainability of Swamp Buffalo. Open Agriculture 2023; 8: 2022022. DOI: https://doi.org/10.1515/opag-2022-0224

Mohd Azmi AF, Ahmad H, Mohd Nor N, Goh YM, Zamri-Saad, M, Abu Bakar, MZ et al. The impact of feed supplementations on Asian buffaloes: A review. Animals 2021; 11(7): 2033. DOI: https://doi.org/10.3390/ani11072033

Noersidiq A, Yanuarianto O, Fahrullah F, Maslami V, Hasma H. Nutrient Content of Corn Straw (Crude Fibre, Crude Fat and TDN) Caused by Different Doses of Urea. Jurnal Pijar Mipa 2024; 19(4): 673-677. DOI: https://doi.org/10.29303/jpm.v19i4.6643

Ervinta E, Mirwandhono RE, Ginting N, Simanullang B. Fermentation by Eco Enzyme on Nutritional Content of Rice Straw, Corn Straw, and Oil Palm Fronds. Jurnal Peternakan Integratif 2020; 8(3): 211-220. DOI: https://doi.org/10.32734/jpi.v8i3.12532

Ginting N, Damanik M. The Study of Sustainable Biodesinfectants for Pig Farms in Samosir Island, Indonesia. In IOP Conference Series: Earth and Environmental Science 2023; 1286(1): 012005. DOI: https://doi.org/10.1088/1755-1315/1286/1/012005

Hong-ya LI, Shu-na LI, Shu-xiang WANG, Quan WANG, Yin-yin XUE, Bao-cheng ZHU. Degradation of Lignocellulose in the Corn Straw by Bacillus Amyloliquefaciens MN-8. Chinese Journal of Applied Ecology/Yingyong Shengtai Xuebao 2015; 26: 5.

Hungate RE. The Rumen and Its Microbes 2013.

Ginting N, Hasnudi H, Prayitno L. Dilution of Eco Enzyme and Antimicrobial Activity Against Staphylococcus aureus. JITRO (Jurnal Ilmu dan Teknologi Peternakan Tropis) 2022; 9(1): 123-128.

Arun C, Sivashanmugam P. Investigation of biocatalytic potential of garbage enzyme and its influence on stabilization of industrial waste activated sludge. Process Saf Environ Prot 2015; 94: 471-478. DOI: https://doi.org/10.1016/j.psep.2014.10.008

Rahman S, Haque I, Goswami RCD, Barooah P, Sood K, Choudhury B. Characterization and FPLC analysis of garbage enzyme: biocatalytic and antimicrobial activity. Waste and Biomass Valorization 2021; 12: 1293-302. DOI: https://doi.org/10.1007/s12649-020-00956-z

Mohamed R, Chaudhry AS. Methods to Study Degradation of Ruminant Feeds. Nutrition Research Reviews 2008; 21(1): 68-81. DOI: https://doi.org/10.1017/S0954422408960674

Prihantoro I, Toharmat T, Evvyernie D, Abdullah L. Ability of fiber bacteria isolated from buffalo rumen in digesting various sources of forage. Jurnal Ilmu Ternak dan Veteriner 2012; 17: 3189-200. DOI: https://doi.org/10.14334/jitv.v17i3.699

Hu Y, He Y, Gao S, Liao Z, Lai T, Zhou H, Chen Q, Li L, Gao H, Lu W. The effect of a diet based on rice straw co-fermented with probiotics and enzymes versus a fresh corn Stover-based diet on the rumen bacterial community and metabolites of beef cattle. Scientific Reports 2020; 10(1): 10721. DOI: https://doi.org/10.1038/s41598-020-67716-w

Natsir A, Syahrir S, Nadir M, Mujnisa A, Purnomo N. Biostarter Formulated from the Rumen Bacteria of Buffalo Effectively Changes the Chemical Composition of the Rice Straw. In IOP Conference Series: Earth and Environmental Science 2020; 492(1): 012003. IOP Publishing. DOI: https://doi.org/10.1088/1755-1315/492/1/012003

IBM SPSS Statistics 29 Step by Step: A Simple Guide and Reference. Routledge.

Ghiasi SE, Gheibipour M, Motamedi H, Dar MA. Screening and Evaluation of the Ruminal Cellulolytic Bacteria and their Potential Application as Probiotics. Iranian Journal of Microbiology 2024; 16: 3389. DOI: https://doi.org/10.18502/ijm.v16i3.15796

Kumar VP, Sridhar M. Study on Production of Microbial Lignolytic Enzymes for Deconstruction of Lignocellulosic Biomass for Feeding Ruminants: Current Trends and Future Research Aspects in Agriculture and Veterinary Science 2021; 3. https://www.researchgate.net/publication/ 355101906_Study_on_Production_of_Microbial_Lignolytic_Enzymes_for_Deconstruction_of_Lignocellulosic_Biomass_for_Feeding_Ruminants_Current_Trends_and_Future_Prospects?enrichId=rgreq-57596ad05e317ddb2c66ebcfc8cf34a4-XXX&enrichSource=Y292ZXJQYWdlOzM1NTEwMTkwNjtBUzoxMTM4ODYxNDMwMzE3MDU3QDE2NDg1MzcxMzIzMjc%3D&el=1_x_2&_esc=publicationCoverPdf.

Rasool G, Irfan M. The Role of Microbial Diversity in Lignocellulosic Biomass Degradation: A Biotechnological Perspective. ChemBioEng Reviews 2024; 11(3): 613-635. DOI: https://doi.org/10.1002/cben.202300073

Guo W, Guo XJ, Zhu BC, Guo YY, Zhou X. In situ degradation, ruminal fermentation, and the rumen bacterial community of cattle fed corn stover fermented by lignocellulolytic microorganisms. Animal Feed Science and Technology 2019; 248: 10-19. DOI: https://doi.org/10.1016/j.anifeedsci.2018.07.007

Welkie DG, Stevenson DM, Weimer PJ. ARISA Analysis of Ruminal Bacterial Community Dynamics in Lactating Dairy Cows During the Feeding Cycle. Anaerobe 2010; 16(2): 94-100. DOI: https://doi.org/10.1016/j.anaerobe.2009.07.002

Zhong H, Zhou J, Wang F, Wu W, Xiong H, Yin H, et al. Isolation and identification of Ligninolytic Bacterium (Bacillus cereus) from Buffalo (Bubalus bubalis) Rumen and its Effects on the Fermentation Quality, Nutrient Composition, and Bacterial Community of Rape Silage. Frontiers in Microbiology 2023; 14: 1103652. DOI: https://doi.org/10.3389/fmicb.2023.1103652

Xiaokang Lv, Qiao Z, Chen C, Hua J, Zhou C. Exploration of Multi-Source Lignocellulose-Degrading Microbial Resources and Bioaugmentation Strategies: Implications for Rumen Efficiency. Animals 2025; 15(13): 1920. DOI: https://doi.org/10.3390/ani15131920

Zhang S, Dong Z, Shi J, Yang C, Fang Y, Chen G, et al. Enzymatic Hydrolysis of Corn Stover Lignin by Laccase, Lignin Peroxidase, and Manganese Peroxidase. Bioresour Technol 2022; 361: 127699. DOI: https://doi.org/10.1016/j.biortech.2022.127699

Ríos-Ríos KL, Dejonghe W, Vanbroekhoven K, Rakotoarivonina H, Rémond C. Enzymatic Production of Xylo-oligosaccharides from Destarched Wheat Bran and the Impact of Their Degree of Polymerization and Substituents on Their Utilization as a Carbon Source by Probiotic Bacteria. J Agric Food Chem 2021; 69: 13217–13226. DOI: https://doi.org/10.1021/acs.jafc.1c02888

Xu J, Wang X, Niu H. Effects of corn straw-based fermented total mixed rations supplemented with exogenous cellulase on growth performance, digestibility, and rumen fermentation in growing beef cattle. Animal Bioscience 2024; 38(2): 293. DOI: https://doi.org/10.5713/ab.24.0246

Chen B, Liu J, Liu M, Zhang H, Li X, Tian C, et al. Synergistic Effect of Microorganisms and Enzymes on Nutritional Value of Corn Stover and Wheat Straw. Fermentation 2025; 11(4): 210. DOI: https://doi.org/10.3390/fermentation11040210

Kala A, Kamra DN, Kumar A, Agarwal N, Chaudhary LC, Joshi CG. Impact of Levels of Total Digestible Nutrients on Microbiome, Enzyme Profile, and Degradation of Feeds in Buffalo Rumen. PloS One 2017; 12(2): e0172051. DOI: https://doi.org/10.1371/journal.pone.0172051

Malik PK. Trivedi S, Kolte AP, Sejian V, Mech A, Biswas S, et al. Comparative Assessment of Rumen Microbial Diversity in Cattle and Buffaloes. In Climate Change and Livestock Production: Recent Advances and Future Perspectives 2022; 223-238. DOI: https://doi.org/10.1007/978-981-16-9836-1_19

Wang X, Song J, Liu Z, Zhang G, Zhang Y. Fermentation Quality and Microbial Community of Corn Stover or Rice Straw Silage Mixed with Soybean Curd Residue. Animals 2022; 12(7): 919. DOI: https://doi.org/10.3390/ani12070919

Yu P, Christensen DA, McKinnon JJ. In situ Rumen Degradation Kinetics of Timothy and Alfalfa as Affected by Cultivar and Stage of Maturity. Can J Anim Sci 2004; 84: 255-263. DOI: https://doi.org/10.4141/A03-116

Maftukhah S. Cellulase Enzyme Production Using Solid State Fermentation Method From Waste–A Review. Jurnal Keilmuan dan Aplikasi Teknik 2019; 6(2): 22. DOI: https://doi.org/10.33592/unistek.v6i2.197

Istiqomah L, Cahyanto MN, Zuprizal Z. Xylanase Production by Trichoderma Virens MLT2J2 Under Solid-State Fermentation Using Corn Cob as a Substrate. Biodiversitas Journal of Biological Diversity 2022; 23: 12. DOI: https://doi.org/10.13057/biodiv/d231251

Samosir Regency Agriculture Office. Interim Livestock Development Report Statistics of Samosir Regency 2025.

Abubakar MS, Zamri-Saad M. Clinico-Pathological Changes in Buffalo Calves Following Oral Exposure to Pasteurella Multocida B:2. Basic and Applied Pathology 2011; 4(4): 130- 135. DOI: https://doi.org/10.1111/j.1755-9294.2011.01113.x

Khan A, Saleemi MK, Khan MZ, Gul ST, Irfan M, Qamar MS. Hemorrhagic Septicemia in Buffalo (Bubalus bubalis) Calves Under Sub-Tropical Conditions in Pakistan. Pakistan Journal of Zoology 2011; 43: 2. https://www.researchgate.net/ publication/241685040

Lestari TD, Khairullah AR, Damayanti R, Mulyati S, Rimayanti R, Hernawati T, et al. Hemorrhagic septicemia: A major threat to livestock health. Open Veterinary Journal 2025; 15(2): 519. DOI: https://doi.org/10.5455/OVJ.2025.v15.i2.3

Downloads

Published

2025-09-14

How to Cite

Ginting, N. ., Mirwandhono, R. E. ., Purba, M. A. ., Nasution, M. A. ., Saragih, W. F. ., Ketaren, N. br. ., & Samsudin, A. A. . (2025). Innovative Use of Endogenous Enzymes to Enhance Silage Chemical Quality of Corn Straw for Buffalo (Bubalusbubalis) Feed in Samosir Island. Journal of Buffalo Science, 14, 131–140. https://doi.org/10.6000/1927-520X.2025.14.16

Issue

Section

Articles