Gas Chromatographic Mass Spectrometric Analysis of Estrus Specific Volatile Compounds in Buffalo Vaginal Mucus after Initial Sexual Foreplay

Authors

  • kandasamy karthikeyan Centre for Pheromone Technology, Department of Animal Science, Bharathidasan University, Tiruchirappalli 620 024, Tamilnadu, India
  • Govindaraju Archunan Department of Biotechnology and Genetic engineering, Bharathidasan University, Tiruchirappalli 620 024, Tamilnadu, India

DOI:

https://doi.org/10.6000/1927-520X.2013.02.01.1

Keywords:

Estrus, vaginal mucus, Saliva, Buffalo, Oleic acid and GC-MS

Abstract

Vaginal fluid from buffaloes in estrus, non-estrus condition and saliva from bulls that have licked vaginal mucus were collected. The samples were analysed by gas chromatography-mass spectrometry (GC-MS) in order to evaluate the qualitative differences between the volatile profiles of vaginal mucus and saliva to assess their potential value in buffalo bio-communication. Fifteen different organic compounds were detected. The chemical profiles of estrus vaginal fluid were distinguished significantly by the presence of specific substance, 9-octadecenoic acid (oleic acid) that was not present in non-estrus phase. The results are consistent with the view that the oleic acid acts as chemosignal in a number of mammalian species. Based on the result it is concluded that the volatile substance present in buffalo vaginal fluid may act as a chemical stimulant.

Author Biography

Govindaraju Archunan, Department of Biotechnology and Genetic engineering, Bharathidasan University, Tiruchirappalli 620 024, Tamilnadu, India

Department of Animal Science

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Published

2013-03-22

How to Cite

karthikeyan, kandasamy, & Archunan, G. (2013). Gas Chromatographic Mass Spectrometric Analysis of Estrus Specific Volatile Compounds in Buffalo Vaginal Mucus after Initial Sexual Foreplay. Journal of Buffalo Science, 2(1), 1–7. https://doi.org/10.6000/1927-520X.2013.02.01.1

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