Molecular Characterisation of an Arcanobacterium sp. Isolate from a Buffalo (Bubalus bubalis)

Authors

  • Siti Gusti Ningrum Universitas Wijaya Kusuma Surabaya, Faculty of Veterinary Medicine, Jl. Dukuh Kupang XXV No.54, Dukuh Kupang, Kec. Dukuhpakis, Surabaya, 60225, Indonesia
  • Antonia Kreitlow Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany
  • Christoph Lämmler Institute of Hygiene and Infectious Diseases of Animals, Justus-Liebig-Universität Giessen, Frankfurter Straße 85-89, 35392 Gießen, Germany
  • Geoffrey Foster SRUC Veterinary Services, RAVIC, 9 Inverness Campus, Inverness IV2 5NA, United Kingdom
  • Maria Borowiak German Federal Institute for Risk Assessment (BfR), Department for Biological Safety, 12277 Berlin, Germany
  • Peter Wragg Animal Health and Veterinary Laboratories Agency, Penrith Regional Laboratory, Merrythought, Calthwaite, Penrith CA11 9RR, UK
  • Madeleine Plötz Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany
  • Amir Abdulmawjood Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany https://orcid.org/0000-0001-5189-8381

DOI:

https://doi.org/10.6000/1927-520X.2024.13.17

Keywords:

Arcanobacterium sp., buffalo, 16S dRNA, ISR, rpoB, gap

Abstract

The objective of the current investigation was to provide a comprehensive characterisation of an Arcanobacterium isolate derived from Bubalus bubalis. The confirmation of the species identity of Arcanobacterium sp. 15/M226/2/9 in this study was achieved by examining phenotypic characteristics and phylogenetic analysis. These analyses involved determining hemolysis on Columbia sheep blood agar and biochemical parameters using the Api-Coryne test kit, MALDI-TOF MS, and partial sequencing of the universal gene encompassing the 16S ribosomal RNA (rRNA) gene, the 16S-23S rDNA intergenic spacer region (ISR), and the two housekeeping genes rpoB and gap. Species classification based on sequencing of the 16S rRNA gene and the ISR revealed a sequence identity of 98.7% each between Arcanobacterium sp. 15/M226/2/9 and A. bovis reference strain C605018/01/1T. In addition, for the other target genes, rpoB and gap, the concordance was 96.6% and 98.8%, respectively. The present study showed that 15/M226/2/9 isolated from B. bubalis could be a novel species.

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Published

2024-12-03

How to Cite

Ningrum, S. G. ., Kreitlow, A. ., Lämmler, C. ., Foster, G. ., Borowiak, M. ., Wragg, P. ., Plötz, M. ., & Abdulmawjood, A. . (2024). Molecular Characterisation of an Arcanobacterium sp. Isolate from a Buffalo (Bubalus bubalis). Journal of Buffalo Science, 13, 150–157. https://doi.org/10.6000/1927-520X.2024.13.17

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Vol. 13 (2024)

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