The Anti-Microbial Properties of Triticum aestivum (Wheat Grass) Extract
DOI:
https://doi.org/10.6000/1927-3037.2015.04.03.1Keywords:
Triticum aestivum, Antimicrobial activity, Solvent extraction, Bioactive molecule, SEMAbstract
Wheat grass, one of the members of Poaceae family, has been considered for very efficient therapeutic drugs. Current study was aimed at evaluation of antimicrobial properties of wheat grass extracts. The 7th., 14th., and 21st. day wheat grass extracts of five different solvents (water, ethanol, methanol, ethyl acetate and hexane) were assayed for antimicrobial activity using turbidity tests. All these extracts showed antibacterial activity against seven food borne pathogens. Amongst them hexane extracts from 7th day old wheat grass showed maximum antibacterial activity especially more against Yersinia enterocolitica and Listeria monocytogenes. The HPLC purified extract was observed to create pores on the cell wall of the bacterial cells as observed under Scanning Electron Microscope and also influenced flattening and shrinkage of bacterial cells indicating probable effect on the membrane of the pathogenic bacteria.
References
Forgionne GA. Bovine cartilage, coenzyme Q10, and wheat grass therapy for primary peritoneal cancer. J Altern Complement Med 2005; 11(1): 161-5. http://dx.doi.org/10.1089/acm.2005.11.161
Meyerowitz S. Wheat grass: nature's finest medicine. Sprout House 1992. ISBN 13: 9781878736970
Ashok SA. Phytochemical and Pharmacological Screening of Wheatgrass Juice (Triticum aestivum L.). International Journal of Pharmaceutical Sciences Review and Research 2011; 9: 159-164.
Padalia S, Drabu S, Raheja I, Gupta A, Dhamija M. Multitude potential of wheatgrass juice (Green Blood): An overview. Chronicles of Young Scientists 2010; 1 (2): 23-28.
Falcioni G, Fedeli D, Tiano L, Calzuola I, Mancinalli L, Gianfranceschi G. Antioxidant activity of wheat sprouts extract in vitro: Inhibition of DNA oxidative damage. Journal of Food Science 2002; 67(8): 2918-2922. http://dx.doi.org/10.1111/j.1365-2621.2002.tb08838.x
Kulkarni SD, Tilak JC, Acharya R, Rajurkar NS, Devasagayam TPA, Reddy AVR. Evaluation of the Antioxidant Activity of Wheatgrass (Triticum aestivum L.) as a Function of Growth under Different Conditions. Phytotherapy Research 2006; 20: 218-227. http://dx.doi.org/10.1002/ptr.1838
Iyer U, Sharma M, Dhruv S, Mani U. Glycemic and lipemic response of wheatgrass incorporated recipes. Toxicology 2010; 4(1): 161-164
Pallavi K, Kumarswammy G, Shruthi. Pharmacognostic investigation and antibacterial activity of Triticum aestivum. Journal of Pharmacy Research 2011; 4(10): 3355-3359
Das A, Raychaudhuri U, Chakraborty R. Antimicrobial effect of edible plant extract on the growth of some foodborne bacteria including pathogens. Nutrafoods 2012; 12: 83-88. http://dx.doi.org/10.1007/s13749-012-0033-z
Weckesser S, Engel K, Haarhaus BS, Wittmer A, Plez K, Schempp CM. Screening of plant extracts for antimicrobial activity against bacteria and yeasts with dermatological relevance. Phytomedicine 2006; 14: 508-516. http://dx.doi.org/10.1016/j.phymed.2006.12.013
Das K, Tiwari RKS, Shrivastava DK. Techniques for evaluation of medicinal plant products as antimicrobial agent: Current methods and future trends. Journal of Medicinal Plants Research 2010; 4(2): 104-111.
Cowan MM. Plant Products as Antimicrobial Agents. Clinical Microbiology Reviews 1999; 12(4): 564-582.
Smullen J, Finney M, Storey DM, Foster HA. Prevention of artificial dental plaque formation in vitro by plant extracts. Journal of Applied Microbiology 2012; 113(4): 964-73. http://dx.doi.org/10.1111/j.1365-2672.2012.05380.x
Ramakrishnan V, Narayan B, Halami PM. Combined effect of enterocin and lipase from Enterococcus faecium NCIM5363 against food borne pathogens: mode of action studies. Current Microbiology 2012; 65(2): 162-9. http://dx.doi.org/10.1007/s00284-012-0138-z
Sarker SD, Nahar L, Kumarasamy Y. Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods (San Diego, Calif.) 2007; 42(4): 321-4. http://dx.doi.org/10.1016/j.ymeth.2007.01.006
Smith BH. Generalization of spatially variant apodization to noninteger Nyquist sampling rats. IEEE Trans Image Process 2000; 9: 1088-1093. http://dx.doi.org/10.1109/83.846250
Rana S, Kamboj JK, Gandhi V. Living life the natural way – Wheatgrass and Health, Functional Foods in Health and Disease 2011; 1(11): 444-456.
Ben AE, Goldin E, Wengrower D, Stamper A, Kohn R, Berry E. Wheat grass juice in the treatment of active distal ulcerative colitis: a randomized double-blind placebo-controlled trial. Scand J Gastroenterol 2002; 37: 444-449. http://dx.doi.org/10.1080/003655202317316088
de Vogel J, Jonker-Termont DS, Katan MB, van der Meer R. Natural chlorophyll but not chlorophyllin prevents heme-induced cytotoxic and hyperproliferative effect in rat colon. J Nutr 2005; 135(8): 1995-2000.
Ferruzzia MG, Blakesleeb J. Digestion, absorption and cancer preventive activity of dietary chlorophyll derivatives. Nutr Res 2007; 27: 1-12. http://dx.doi.org/10.1016/j.nutres.2006.12.003
Meyerowitz S. Wheatgrass Nature's Finest Medicine: The Complete Guide to Using Grass Foods & Juices to Revitalize Your Health. 6th ed. Sproutman Publications 1999; p. 53.
Wheat J, Currie J. Herbal medicine for cancer patients: An evidence based review. The Internet Journal of Alternative Medicine 2008; 5: 28-30.
Das A, Raychaudhuri U, Chakraborty R. Antimicrobial effect of edible plant extract on the growth of some foodborne bacteria including pathogens. Nutrafoods 2012; 12: 83-88. http://dx.doi.org/10.1007/s13749-012-0033-z
Desai TR. 2005. Investigation into the Mechanism of Action and Effects of Triticum Aestivum (Wheat) Grass. Etheses-Saurashtra University library service [webpage]. Available from http://etheses.saurashtrauniversity.edu/id/eprint/188
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