Application of Membrane Technology to Slaughterhouse Blood to Produce Edible Powdered Protein Mixture

Authors

  • Maria I. Kokkora Department of Biosystems Engineering, School of Agricultural Technology, Technological Educational Institute (TEI) of Larissa, 41110, Larissa, Greece
  • Konstantinos B. Petrotos Department of Biosystems Engineering, School of Agricultural Technology, Technological Educational Institute (TEI) of Larissa, 41110, Larissa, Greece
  • Paschalis E. Gkoutsidis Department of Biosystems Engineering, School of Agricultural Technology, Technological Educational Institute (TEI) of Larissa, 41110, Larissa, Greece
  • Christos Mpoulmpos Technological Research Center of Thessalia, TEI of Larissa, 41110, Larissa, Greece

DOI:

https://doi.org/10.6000/1929-6037.2012.01.01.5

Keywords:

Animal blood, ultrafiltration, microfiltration, freeze drying, red protein powder mixture, white protein powder mixture.

Abstract

Animal blood generated from slaughtered animals is often released into the environment resulting in significant pollution and also in the loss of a valuable protein source. This study aimed at developing a procedure that will allow for utilizing animal blood for protein powders production on an industrial scale. To meet this goal, hygienically collected animal blood was first treated with membrane technology: microfiltration (MF) or ultrafiltration (UF). A ceramic MF membrane and a PCI UF membrane were used. Average MF flux was 6.62 kg h-1 m-2 at transmembrane pressure of 2.5 bar. Average UF flux was 3.55 kg h-1 m-2 at transmembrane pressure of 4 bar. MF succeeded in separating the blood plasma proteins (permeate) from the red cell fraction (retentate). UF concentrated both the red cell and blood plasma proteins in a single sample (retentate). The volume ratio of retentate to permeate was 10:14 and 14:10, for the MF and UF, respectively. A membrane cleaning regime was developed. The treated blood fractions were then freeze dried and red and white protein powders were produced successfully. The potential of a SME (small-medium enterprise) to apply this procedure into practice is presented.

References

Dart MC. Treatment of wastewaters from the meat industry. Proc Biochem 1974; 9(Pt 5): 11-14.

Hyun C-K, Shin H-K. Utilization of bovine blood plasma proteins for the production of angiotensin I converting enzyme inhibitory peptides. Proc Biochem 2000; 36(Pt 1-2): 65-71. http://dx.doi.org/10.1016/S0032-9592(00)00176-X DOI: https://doi.org/10.1016/S0032-9592(00)00176-X

Gómez-Juárez C, Castellanos R, Ponce-Noyola T, Calderón V, Figueroa J. Protein recovery from slaughterhouse wastes. Bioresource Technol 1999; 70(Pt 2): 129-33. DOI: https://doi.org/10.1016/S0960-8524(99)00030-9

Moure F, Rendueles M, Díaz M. Coupling process for plasma protein fractionation using ethanol precipitation and ion exchange chromatography. Meat Sci 2003; 64: 391-98. http://dx.doi.org/10.1016/S0309-1740(02)00205-X DOI: https://doi.org/10.1016/S0309-1740(02)00205-X

Moure F, Rendueles M, Díaz M. Bovine plasma protein fractionation by ion exchange chromatography. Bioprocess Biosyst Eng 2004; 27:17-24. http://dx.doi.org/10.1007/s00449-004-0372-2 DOI: https://doi.org/10.1007/s00449-004-0372-2

Ledward DA, Lawrie RA. Recovery and utilisation of by-product proteins of the meat industry. J Chem Tech Biotechnol 1984; 34(Pt B): 223-28. DOI: https://doi.org/10.1002/jctb.280340312

Torres MR, Marı́n FR, Ramos AJ, Soriano E. Study of operating conditions in concentration of chicken blood plasma proteins by ultrafiltration. J Food Eng 2003; 54(Pt 3): 215-19. DOI: https://doi.org/10.1016/S0260-8774(01)00204-7

Del Hoyo P, Rendueles M, Díaz M. Effect of processing on functional properties of animal blood plasma. Meat Sci 2008; 78: 522-28. http://dx.doi.org/10.1016/j.meatsci.2007.07.024 DOI: https://doi.org/10.1016/j.meatsci.2007.07.024

Fernando T. Concentration of animal blood by ultrafiltration. Biotechnol Bioeng 1981; 23(Pt 1): 19-27. http://dx.doi.org/10.1002/bit.260230103 DOI: https://doi.org/10.1002/bit.260230103

Jönsson A-S, Trägårdh G. Ultrafiltration applications. Desalination 1990; 77: 135-79. DOI: https://doi.org/10.1016/0011-9164(90)85024-5

Wojciechowski C, Chwojnowski A, Dubziński K, Łukowska E. Evaluation of separation characteristic of poly sulfone membranes modified by polymer solvents etching. Desalin Water Treat 2011; 35: 263-70. http://dx.doi.org/10.5004/dwt.2011.2487 DOI: https://doi.org/10.5004/dwt.2011.2487

Selmane D, Christophe V, Gholamreza D. Extraction of proteins from slaughterhouse by-products: Influence of operating conditions on functional properties. Meat Sci 2008; 79: 640-47. http://dx.doi.org/10.1016/j.meatsci.2007.10.029 DOI: https://doi.org/10.1016/j.meatsci.2007.10.029

Belhocine D, Grib H, Abdessmed D, Comeau Y, Mameri N. Optimization of plasma proteins concentration by ultrafiltration. J Membrane Sci 1998; 142: 159-71. http://dx.doi.org/10.1016/S0376-7388(97)00303-7 DOI: https://doi.org/10.1016/S0376-7388(97)00303-7

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Published

2012-10-05

How to Cite

Kokkora, M. I., Petrotos, K. B., Gkoutsidis, P. E., & Mpoulmpos, C. (2012). Application of Membrane Technology to Slaughterhouse Blood to Produce Edible Powdered Protein Mixture. Journal of Membrane and Separation Technology, 1(1), 35–42. https://doi.org/10.6000/1929-6037.2012.01.01.5

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