The Cause for Heart Disease and Strokes
DOI:
https://doi.org/10.6000/1929-5634.2013.02.02.7Keywords:
Oxysterols, phospholipids, cholesterol, calcium, oxidationAbstract
There has been no solution to heart disease to date. I obtained discarded veins from bypass (CABG) surgeries and subjected them to phospholipid analysis. We also obtained arterial cells from human umbilical cords and cultured them with a decreasing concentration of either cholesterol or oxidized cholesterol. Patients undergoing CABG surgery and aging swine had significantly higher levels of sphingomyelin in their arterial cells than arterial cells from human umbilical cords. Oxidized low-density lipoprotein (OxLDL) and oxysterols further contribute to atherosclerosis by increasing the synthesis of thromboxane in platelets, a clotting factor. When we incubated arterial cells with cholesterol that had not been oxidized, even at twelve times the concentration of the oxidized cholesterols we used, there was no effect on sphingomyelin content, this shows that cholesterol itself is not the reason for heart disease, and has to be oxidized in order to cause harm. My study indicated that atherosclerosis is due to a diet that contains a high level of oxysterols. Normal levels of oxysterols in the plasma will not increase sphingomyelin levels. Removing oxidized fat from the diet should be considered as a therapeutic measure for atherosclerosis. Ancel Keys, who some consider the father of the cholesterol-heart disease hypothesis said in 1997: "There's no connection whatsoever between the cholesterol in food and cholesterol in the blood."
References
Staprans I, Xian-Mang P, Rapp JH, Feingold KR. Oxidized Cholesterol in the Diet Accelerates the Development of Aortic Atherosclerosis in Cholesterol-Fed Rabbits. Arteriosclerosis, Thrombosis, and Vascular Biology 1998; 18: 977-83. http://dx.doi.org/10.1161/01.ATV.18.6.977 DOI: https://doi.org/10.1161/01.ATV.18.6.977
De Bakey ME, Diethrich EB, Garrett HE, McCutchen JJ. Surgical treatment of cerebrovascular disease. Postgrad Med 1967; 42: 218-30. DOI: https://doi.org/10.1080/00325481.1967.11696269
Kummerow FA, Przybylski R, Wasowicz E. Changes in arterial membrane lipid composition may precede growth factor influence in the pathogenesis of atherosclerosis. Artery 1994; 21: 63-75.
Zhou Q, Smith T, Kummerow FA. Cytotoxicity of Oxysterols on Cultured Smooth Muscle Cells from Human Umbilical Arteries. Soc Exper Biol Med 1993; 202: 75-80. http://dx.doi.org/10.3181/00379727-202-43514 DOI: https://doi.org/10.3181/00379727-202-43514
Zhou Q, Kummerow FA. Effect of 27-hydroxycholesterol on cellular sphingomyelin synthesis and Ca++ content in cultured smooth muscle cells. Biomed Environ Sci 1997; 10: 369-76.
Zhou Q, Kummerow FA. Alterations of Ca++ Uptake and Lipid Content in Cultured Human Arterial Smooth Muscle Cells Treated with 26-Hydrocholesterol. Artery 1994; 21(4): 182-92.
Javitt NB. 26-Hydroxycholesterol: synthesis, metabolism, and biologic activities. J Lipid Res 1990; 31: 1527-33. DOI: https://doi.org/10.1016/S0022-2275(20)42337-5
Shah DO, Schulman JH. Interaction of calcium ions with lecithin and sphingomyelin monolayers. Lipids 1967; 2: 21-27. http://dx.doi.org/10.1007/BF02531995 DOI: https://doi.org/10.1007/BF02531995
Kummerow FA, Cook LS, Wasowicz E, Jelen H. Changes in the phospholipid composition of the arterial cell can result in severe atherosclerotic lesions. J Nutr Biochem 2001; 12: 602-607. http://dx.doi.org/10.1016/S0955-2863(01)00181-4 DOI: https://doi.org/10.1016/S0955-2863(01)00181-4
Smith LL. Cholesterol autoxidation. New York: Plenum 1981. http://dx.doi.org/10.1007/978-1-4757-9691-9 DOI: https://doi.org/10.1007/978-1-4757-9691-9
Kummerow FA, Olinescu RM, Fleischer L, Handler B, Shinkareva SV. The relationship of oxidized lipids to coronary artery stenosis. Atherosclerosis 2000; 149: 181-90. http://dx.doi.org/10.1016/S0021-9150(99)00378-0 DOI: https://doi.org/10.1016/S0021-9150(99)00378-0
Mahfouz MM, Kummerow FA. Oxysterols and TBARS are among the LDL oxidation products which enhance thromboxane A2 synthesis by platelets. Prostaglandins Other Lipid Mediat 1998; 56: 197-17. http://dx.doi.org/10.1016/S0090-6980(98)00056-2 DOI: https://doi.org/10.1016/S0090-6980(98)00056-2
Mahfouz MM, Kummerow FA. Oxidized low-density lipoprotein (LDL) enhances thromboxane A(2) synthesis by platelets, but lysolecithin as a product of LDL oxidation has an inhibitory effect. Prostaglandins Other Lipid Mediat 2000; 62: 183-200. http://dx.doi.org/10.1016/S0090-6980(00)00078-2 DOI: https://doi.org/10.1016/S0090-6980(00)00078-2
Mahfouz MM, Zhou Q, Kummerow FA. Inhibition of prostacyclin release by cigarette smoke extract in endothelial cells is not related to enhanced superoxide generation and NADPH-oxidase activation. J Environ Pathol Toxicol Oncol 2006; 25: 585-95. http://dx.doi.org/10.1615/JEnvironPatholToxicolOncol.v25.i3.30 DOI: https://doi.org/10.1615/JEnvironPatholToxicolOncol.v25.i3.30
Kummerow FA, Mahfouz MM, Zhou Q. Trans fatty acids in partially hydrogenated soybean oil inhibit prostacyclin release by endothelial cells in presence of high level of linoleic acid. Prostaglandins Other Lipid Mediat 2007; 84: 138-53. http://dx.doi.org/10.1016/j.prostaglandins.2007.07.001 DOI: https://doi.org/10.1016/j.prostaglandins.2007.07.001
Kummerow FA. The negative effects of hydrogenated trans fats and what to do about them. Atherosclerosis 2009; 205: 458-65. http://dx.doi.org/10.1016/j.atherosclerosis.2009.03.009 DOI: https://doi.org/10.1016/j.atherosclerosis.2009.03.009
Vane JR, Bakhle YS, Botting RM. Cyclooxygenase 1 and 2. Annu Rev Pharmacol Toxicol 1998; 38: 98-20. http://dx.doi.org/10.1146/annurev.pharmtox.38.1.97 DOI: https://doi.org/10.1146/annurev.pharmtox.38.1.97
Vane JR, Mitchell JA, Appleton I. Inducible isoforms of cyclooxygenase and nitric-oxide synthase in inflammation. Proc Natl Acad Sci USA 1994; 91: 2046-50. http://dx.doi.org/10.1073/pnas.91.6.2046 DOI: https://doi.org/10.1073/pnas.91.6.2046
Kummerow FA. Improving hydrogenated fat for the world population. Prevention Control 2005; 1: 157-64. http://dx.doi.org/10.1016/j.precon.2005.05.003 DOI: https://doi.org/10.1016/j.precon.2005.05.003
Vesper HW, Kuiper HC, Mirel, LB, Johnson CL, Pirkle JL. Research Letter: Levels of Plasma trans-Fatty Acids in Non-Hispanic White Adults in the United States in 2000 and 2009. JAMA 2012; 307(6): 562-63. http://dx.doi.org/10.1001/jama.2012.112 DOI: https://doi.org/10.1001/jama.2012.112
Pryor WA. The role of free radical reactions in biological systems. New York: Academic Press 1977. DOI: https://doi.org/10.1016/B978-0-12-566501-8.50008-0
Maddox T. Heart disease and sudden cardiac death. Retrieved from http://www/webmd.com/heart-disease/guide/ sudden-cardiac-death; Accessed 1 May 2012.
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