Platelets Mitochondrial Function Depends on Coenzyme Q10 Concentration in Human Young, Not in Elderly Subjects

Anna Gvozdjáková; Zuzana Sumbalová; Jarmila Kucharská; Anežka Chládeková; Zuzana Rausová; Olga Vancová; Mária Kubalová; Zuzana Kuzmiaková; Michal Nemec; Olga  Ulicná; Viliam Mojto

doi:10.6000/1929-5634.2018.07.03.1

Authors

Anna Gvozdjáková Pharmacobiochemical Laboratory of 3rd Medical Department, Medical Faculty, Comenius University in Bratislava Sasinkova 4, 811 08 Bratislava, Slovakia
Zuzana Sumbalová Pharmacobiochemical Laboratory of 3rd Medical Department, Medical Faculty, Comenius University in Bratislava Sasinkova 4, 811 08 Bratislava, Slovakia
Jarmila Kucharská Pharmacobiochemical Laboratory of 3rd Medical Department, Medical Faculty, Comenius University in Bratislava Sasinkova 4, 811 08 Bratislava, Slovakia
Anežka Chládeková 3rd Medical Department, Medical Faculty, Comenius University in Bratislava, Slovakia
Zuzana Rausová Pharmacobiochemical Laboratory of 3rd Medical Department, Medical Faculty, Comenius University in Bratislava Sasinkova 4, 811 08 Bratislava, Slovakia
Olga Vancová Pharmacobiochemical Laboratory of 3rd Medical Department, Medical Faculty, Comenius University in Bratislava Sasinkova 4, 811 08 Bratislava, Slovakia
Mária Kubalová Pharmacobiochemical Laboratory of 3rd Medical Department, Medical Faculty, Comenius University in Bratislava Sasinkova 4, 811 08 Bratislava, Slovakia
Zuzana Kuzmiaková Pharmacobiochemical Laboratory of 3rd Medical Department, Medical Faculty, Comenius University in Bratislava Sasinkova 4, 811 08 Bratislava, Slovakia
Michal Nemec Institute of Experimental Endocrinology, Biomedical Research Center Slovak Academy of Sciences, Bratislava, Slovakia
Olga Ulicná Pharmacobiochemical Laboratory of 3rd Medical Department, Medical Faculty, Comenius University in Bratislava Sasinkova 4, 811 08 Bratislava, Slovakia
Viliam Mojto 3rd Medical Department, Medical Faculty, Comenius University in Bratislava, Slovakia

DOI:

https://doi.org/10.6000/1929-5634.2018.07.03.1

Keywords:

Platelets, mitochondria, High-Resolution Respirometry, coenzyme Q10, age.

Abstract

Ageing is characterized by a progressive decline in the physiological functions of various organs. Mitochondrial alterations occurring in senescence. Antioxidants, including coenzyme Q₁₀ concentration, fall with ageing and contribute to enhanced oxidative stress age-related diseases. The impairment of platelet mitochondrial function occurs in a broad spectrum of diseases.

The aim of this study was to evaluate mitochondrial function in platelets in elderly and young human controls and correlate it with a concentration of coenzyme Q₁₀. Platelets mitochondrial function was determined by the use of High-Resolution Respirometry method.

We did not find significantly decreased platelet mitochondrial function in elderly subjects. Dependence of platelets mitochondrial respiratory chain function and ATP production at Complex I on a concentration of coenzyme Q₁₀ in platelets and whole blood in young not in elderly human volunteers was documented. This dependence was not found for Complex II in any group. Platelet mitochondrial coenzyme Q₁₀ concentration was insufficient for improving platelet mitochondrial function in elderly human subjects. Recommending supplementation with coenzyme Q₁₀in elderly and aged humans is waranted.

High-Resolution Respirometry method offers a perspective to diagnose mitochondrial energy metabolism which might be useful for further studies in patients with mitochondrial disorders. Our results could contribute to the explanation of platelets mitochondrial function in elderly and aged human subjects.

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