Preparation of Low-Phenylalanine Macro Peptides and Estimation of its Phenylalanine Content by Fluorometric Technique

Authors

  • Elevina Pérez Instituto de Ciencia y Tecnología de Alimentos, Universidad Central de Venezuela. Apartado Postal 47097, Caracas 1041-A, Venezuela
  • Liz Pérez Instituto de Ciencia y Tecnología de Alimentos, Universidad Central de Venezuela. Apartado Postal 47097, Caracas 1041-A, Venezuela
  • Lucrecia Requena Instituto de Ciencia y Tecnología de Alimentos, Universidad Central de Venezuela. Apartado Postal 47097, Caracas 1041-A, Venezuela
  • Antonieta Mahfoud Instituto de Estudios Avanzados Carretera, Nacional Hoyo de la Puerta, Baruta. CP 1070 Caracas Venezuela
  • Carmen Luisa Domínguez Instituto de Estudios Avanzados Carretera, Nacional Hoyo de la Puerta, Baruta. CP 1070 Caracas Venezuela
  • Alejandra Rengel Instituto de Ciencia y Tecnología de Alimentos, Universidad Central de Venezuela. Apartado Postal 47097, Caracas 1041-A, Venezuela
  • Davdmary Cueto Instituto de Ciencia y Tecnología de Alimentos, Universidad Central de Venezuela. Apartado Postal 47097, Caracas 1041-A, Venezuela
  • Romel Guzmán Instituto de Ciencia y Tecnología de Alimentos, Universidad Central de Venezuela. Apartado Postal 47097, Caracas 1041-A, Venezuela
  • Pablo Rodríguez Instituto de Ciencia y Tecnología de Alimentos, Universidad Central de Venezuela. Apartado Postal 47097, Caracas 1041-A, Venezuela
  • Erika Crespo Instituto de Estudios Avanzados Carretera, Nacional Hoyo de la Puerta, Baruta. CP 1070 Caracas Venezuela
  • Katiuska Araujo Instituto de Estudios Avanzados Carretera, Nacional Hoyo de la Puerta, Baruta. CP 1070 Caracas Venezuela
  • Leny Sua Instituto de Estudios Avanzados Carretera, Nacional Hoyo de la Puerta, Baruta. CP 1070 Caracas Venezuela

DOI:

https://doi.org/10.6000/1929-5634.2013.02.03.2

Keywords:

Flours, L-phenylalanine, PKU food, dietary supplementation

Abstract

The aims of the study were to prepare macro peptides low in phenylalanine (Phe) from non-conventional raw materials, and to demonstrate the feasibility of using the fluorometric technique to measure the diminution of their Phe content. Aqueous solution of flours of legumes, and amaranth panicles were used to elaborate the concentrates by using isoelectric precipitation. These protein concentrates, and a whey solution were incubated with proteolytic enzymes to hydrolyze the peptide link at the aromatic amino acids, and then these macro peptides were filtrated through activated charcoal, in order to reduce its phenylalanine concentration. The Phe concentration, of the each prepared macro peptides, was analyzed by using fluorometric technique, and it was later validated by using HPLC. The crude protein contents in the concentrates have varied from 90% in the protein isolate from lentils, 76% in those from the frijol white, and 44% in those from amaranth panicles. Protein concentrates, and whey were hydrolyzed by using the following enzymes: pepsin from the pig gastric mucosa, protease from Aspergillus oryzae, and protease type XIV from Streptomyces griseus. It was determined that the enzymes with the better hydrolysis capacity, were the proteases from S. griseus and A. oryzae. The macro peptides with non-linked phe were filtered through activated charcoal. Reductions of Phe of up to 99% in the second and third filtrate were observed and this reduction was corroborated by using HPLC technique. It was also established the higher sensitivity of the fluorometric method to detect Phe, than the HPLC technique.

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Published

2013-09-30

How to Cite

Pérez, E., Pérez, L., Requena, L., Mahfoud, A., Domínguez, C. L., Rengel, A., Cueto, D., Guzmán, R., Rodríguez, P., Crespo, E., Araujo, K., & Sua, L. (2013). Preparation of Low-Phenylalanine Macro Peptides and Estimation of its Phenylalanine Content by Fluorometric Technique. Journal of Nutritional Therapeutics, 2(3), 145–153. https://doi.org/10.6000/1929-5634.2013.02.03.2

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