Functional Properties of Gelatin Hydrolysate from Salmon Skin (Salmo salar)
DOI:
https://doi.org/10.6000/1929-5634.2014.03.02.7Keywords:
Gelatin hydrolysate, salmon skin, foaming properties, emulsifying properties.Abstract
Gelatin hydrolysate is derived from a hydrolysis of gelatin (denatured collagen) to expose the functional properties of the hydrolysate obtained. This study was carried out to determine functional properties of gelatin hydrolysate from salmon skin as influenced by the degree of enzymatic hydrolysis. Aqueous extraction of gelatin from salmon skin was done at 45oC for 60 minutes. The extracted gelatin was then hydrolysed using 1% alcalase at 54.5oC and pH 8. The functional properties determined were molecular weight, solubility, foaming capacity and stability, emulsifying activity and stability index were analysed. It was found that different time of hydrolysis (5, 15, 45 and 180 minutes) resulted in different degree of hydrolysis (DH) (10, 20, 30 and 40%, respectively). The gelatin hydrolysate at 40% DH showed the highest solubility (p<0.05). However, the foaming and emulsifying properties were the highest at 10% degree of hydrolysis (p<0.05).
References
Wasswa J, Tang J, Gu X, Yuan X. Influence of the extent of enzymatic hydrolysis on the functional properties of protein hydrolysate from grass carp (Ctenopharngodon idella) skin. Food Chemistry 2007; 104: 1698-1704. http://dx.doi.org/10.1016/j.foodchem.2007.03.044 DOI: https://doi.org/10.1016/j.foodchem.2007.03.044
Zague V. A new view concerning the effects of collagen hydrolysate intake on skin properties. Arch Dermatol Res 2008; 300: 479-483. http://dx.doi.org/10.1007/s00403-008-0888-4 DOI: https://doi.org/10.1007/s00403-008-0888-4
Choi SS, Regenstein JM. Physico-chemical and sensory characteristics of fish gelatin. Journal of Food Science 2000; 65: 194-199. http://dx.doi.org/10.1111/j.1365-2621.2000.tb15978.x DOI: https://doi.org/10.1111/j.1365-2621.2000.tb15978.x
Phanturat P, Benjakul S, Visessanguan W, Roytrakul S. Use of pyloric caeca extract from bigeye snapper (Priacanthus macracanthus) for the production of gelatin hydrolysate with antioxidative activity. LWT - Food Science and Technology 2010; 43 (1): 86-97. http://dx.doi.org/10.1016/j.lwt.2009.06.010 DOI: https://doi.org/10.1016/j.lwt.2009.06.010
Liu Q, Kong B, Xiong YL, Xia X. Antioxidant activity and functional properties of porcine plasma protein hydrolysate as influenced by the degree of hydrolysis. Food Chemistry 2010; 118: 403-410. http://dx.doi.org/10.1016/j.foodchem.2009.05.013 DOI: https://doi.org/10.1016/j.foodchem.2009.05.013
Spellman D, McEvoy E, O’Cuinn G, FitzGerald RJ. Proteinase and exopeptidase hydrolysis of whey protein: Comparison of the TNBS, OPA and pH-stat methods for quantification of degree of hydrolysis. International Dairy Journal 2003; 13: 447-453. http://dx.doi.org/10.1016/S0958-6946(03)00053-0 DOI: https://doi.org/10.1016/S0958-6946(03)00053-0
Quaglia GB, Orban E. Influence of enzymatic hydrolysis on structure and emulsifying properties of sardine (Sardina pilchardus) protein hydrolysate. Journal of Food Science 1990; 55: 1571-1573. http://dx.doi.org/10.1111/j.1365-2621.1990.tb03571.x DOI: https://doi.org/10.1111/j.1365-2621.1990.tb03571.x
Shahidi F, Xiao-Quing H, Synowiecki J. Production and characteristics of protein hydrolysates from capelin (Mallotus villosus). Food Chemistry 1995; 53: 285-293. http://dx.doi.org/10.1016/0308-8146(95)93934-J DOI: https://doi.org/10.1016/0308-8146(95)93934-J
Diniz FM, Martin AM. Effects of the extent of enzymatic hydrolysis on functional properties of shark protein hydrolysate. Lebensmittel-Wissenschaftund-Technologie 1997; 30: 266-272. http://dx.doi.org/10.1006/fstl.1996.0184 DOI: https://doi.org/10.1006/fstl.1996.0184
Sathivel S, Bechtel PJ, Crapo S, Reppond KD, Prinnyawiwatkul W. Biochemical and functional properties of herring (Clupea harengus). Journal of Food Science 2003; 68: 2196-2200. http://dx.doi.org/10.1111/j.1365-2621.2003.tb05746.x DOI: https://doi.org/10.1111/j.1365-2621.2003.tb05746.x
Sathivel S, Smiley S, Prinyawiwatkul W, Bechtel PJ. Functional and nutritional properties of red salmon (Oncorhynchus nerka) enzymatic hydrolysates. Journal of Food Science 2005; 70(6): 401–406. http://dx.doi.org/10.1111/j.1365-2621.2005.tb11437.x DOI: https://doi.org/10.1111/j.1365-2621.2005.tb11437.x
Jung S, Murphy PA, Johnson LA. Physicochemical and functional properties of soy protein substrates modified by low levels of protease hydrolysis. J Food Sci 2005; 70: C180-C187. DOI: https://doi.org/10.1111/j.1365-2621.2005.tb07080.x
Sinha R, Radha C, Prakash J, Kaul P. Whey protein hydrolysate: functional properties, nutritional properties and utilization in beverage formulation. Food Chemistry 2007; 101(4): 1484-1491. http://dx.doi.org/10.1016/j.foodchem.2006.04.021 DOI: https://doi.org/10.1016/j.foodchem.2006.04.021
Kolodziejska I, Skierka E, Sadowska M, Kolodziejski W, Niecikowska C. Effect of extracting time and temperature on yielding of gelatin from different fish offal. Food Chemistry 2008; 107: 700-706. http://dx.doi.org/10.1016/j.foodchem.2007.08.071 DOI: https://doi.org/10.1016/j.foodchem.2007.08.071
Yang JI, Ho HY, Chu YJ, Chow CJ. Characteristic and antioxidative activity of retorded gelatin hydrolysate from cobia (Rachycentron canadum) skin. Food Chemistry 2008; 110: 128-136. http://dx.doi.org/10.1016/j.foodchem.2008.01.072 DOI: https://doi.org/10.1016/j.foodchem.2008.01.072
Adler-Nissen J. Determination of the degree of hydrolysis of food protein hydrolysate by trinitrobenzenesulfonic acid. Journal of Agricultural and Food Chemistry 1979; 27: 1256-1262. http://dx.doi.org/10.1021/jf60226a042 DOI: https://doi.org/10.1021/jf60226a042
Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680–685. http://dx.doi.org/10.1038/227680a0 DOI: https://doi.org/10.1038/227680a0
AOAC. Official Methods of Analysis. 2nd Ed. Washington D. C.: Association of Official Analytical Chemists 1990.
Klompong V, Benjakul S, Kantachote D, Shahidi F. Antioxidative activity and functional properties of protein hydrolysate of yellow stripe trevally (Selaroides leptolepis) as influenced by the degree of hydrolysis and enzyme type. Food Chemistry 2007; 102: 1317-1327. http://dx.doi.org/10.1016/j.foodchem.2006.07.016 DOI: https://doi.org/10.1016/j.foodchem.2006.07.016
Pearce KN, Kinsella JE. Emulsifying properties of proteins: Evaluation of a turbidimetric technique. Journal of Agricultural and Food Chemistry 1978; 26: 716-723. http://dx.doi.org/10.1021/jf60217a041 DOI: https://doi.org/10.1021/jf60217a041
Gbogouri GA, Linder M, Fanni J, Parmentier M. Influence of hydrolysis degree on the functional properties of salmon byproduct hydrolysates. Journal of Food Science 2004; 69: 615-622. http://dx.doi.org/10.1111/j.1365-2621.2004.tb09909.x DOI: https://doi.org/10.1111/j.1365-2621.2004.tb09909.x
Weaire D, Hutzler S. The Physics of Foams, Oxford University Press, Oxford; 1999. DOI: https://doi.org/10.1093/oso/9780198505518.001.0001
Manev ED, Nguyen AV. Effects of surfactant adsorption and surface forces on thinning and rupture of foam liquid films. Int J of Mineral Processing 2005; 77: 45. http://dx.doi.org/10.1016/j.minpro.2005.01.003 DOI: https://doi.org/10.1016/j.minpro.2005.01.003
Belitz HD, Grosch W, Schieberle P. Protein. Food Chemistry. Heidelberg: Springer; 2009.
Sikorski ZE. Chemical and functional properties of food components. Taylor, Francis Group: CRC Press; 2007. DOI: https://doi.org/10.1201/9781420009613
Chobert JM, Bertrand-Harb C, Nicolus MG. Solubility and emulsifying properties of caseins and whey proteins modified enzymatically by trypsin. Journal of Agricultural and Food Chemistry 1988; 36: 674-677. DOI: https://doi.org/10.1021/jf00083a002
Nalinanon S, Benjakul S, Visessanguan W, Kishimura H. Improvement of gelatin extraction from bigeye snapper skin using pepsinaided process in combination with protease inhibitor. Food Hydrocolloids 2008; 22 (4): 615-622. http://dx.doi.org/10.1016/j.foodhyd.2007.01.012 DOI: https://doi.org/10.1016/j.foodhyd.2007.01.012
Zhu H, Damodaran S. Effects of calcium and magnesium ions on aggregation of whey protein isolate and its effect on foaming properties. J Agric Food Chem 1994; 42: 856. http://dx.doi.org/10.1021/jf00040a003 DOI: https://doi.org/10.1021/jf00040a003
Phillips LG, Whitehead DM, Kinsella JE. Protein stabilized foams. Structure-function of Food Proteins. New York: Academic Press; 1994. DOI: https://doi.org/10.1016/B978-0-12-554360-6.50011-5
Multilangi WAM, Panyam D, Kilara A. Functional properties of hydrolysates from proteolysis of heat-denatured whey protein isolate. Journal of Food Science 1996; 61: 270-274. http://dx.doi.org/10.1111/j.1365-2621.1996.tb14174.x DOI: https://doi.org/10.1111/j.1365-2621.1996.tb14174.x
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