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Abstract : Differences in Parameters of an Embryo In Vitro Production Program between Cattle (Bos Indicus) and Buffaloes (Bubalus bubalis)
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Abstract: In order to improve production, it is necessary to apply reproductive biotechnologies, including embryo transfer. Due to the management and physiology of the animals and the buffalo production system, the best system is the in vitro production of embryos (IVP). This work aims to compare the results of the (IVP) of cows (Bos indicus) and buffalo (Bubalus bubalis) from animals kept under the same conditions of feeding and handling. This study was conducted in an Argentinan commercial herd located in the province of Corrientes (-27.742859 latitude, -57.773611 longitude) that raise buffaloes and cattle, during the breeding season of 2018 (March-May). Twenty animals of each species were used. Antimullerian hormone (AMH) levels of each animal were determined using ELISA. Standardized protocols were used for oocyte aspiration, maturation, fertilization and culture of the embryos, frozen semen of a single proved bull was used in each species. Information about the number of follicles, oocytes, and embryos was recorded and analyzed individually and grouped by species. The normality of the data was evaluated with the D'Agostino and Shapiro-Wilk tests and the comparisons between species using the Mann Whitney and ANOVA tests. Values are shown as median and range. A p-value <0.05 was="" considered="" statistically="" significant="" the="" amh="" levels="" of="" cows="" were="" 688="" 5="" pg="" ml="" 45="" 3-2394="" and="" buffaloes="" 73="" 8="" 14="" 8-262="" p="" 0="" 001="" differences="" found="" in="" number="" recovered="" oocytes="" 9="" 0-23="" vs="" 4="" 1-11=""> 0.05). There were no significant differences in the number of follicles and the quality of the oocytes. Significant differences were found in the number of oocytes cleaved 4 (0 -17) vs. 0.5 (0-4) and blastocysts/animal 1,5 (0-15) and 0,1 (0-2) l for cows and buffalos respectively. The number of blastocysts in relation to the number of oocytes cleaved did not show statistical significance. The differences in the levels of AMH and the marked differences in the IVP between buffaloes and cattle are confirmed, it is necessary to propose research proposals that explain the differences. Keywords: IVPE, buffaloes, AMH, Differences. |
Abstract : Prevalence and Diffusion of Gastrointestinal Parasite Infections in Swamp Water Buffalo (Bubalus Bubalis) Populations from Marshlands of Iraq
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Abstract: Background and objectives: New information on the epidemiology of gastrointestinal (GI) parasite infection in water buffaloes is crucial for understanding their risk factors and transmission. The objectives of this study were (1) to determine the prevalence of GI parasites in buffaloes in the Marshland areas of southern Iraq, and (2) to evaluate the association of risk factors with the parasitic infections. Keywords: Swamp buffaloes, epidemiology, endoparasites, gastrointestinal parasites, Bubalus bubalis, Marshes. |
Abstract : Study of Population Structure and Genetic Prediction of Buffalo from Different Provinces of Iran using Machine Learning Method
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Abstract: Considering breeding livestock programs to milk production and type traits based on existence two different ecotypes of Iranian’s buffalo, a study carried out to investigate the population structure of Iranian buffalo and validate its classification accuracy according to different ecotypes from Iran (Azerbaijan and North) using data SNP chip 90K by means Support vector Machine (SVM), Random Forest (RF) and Discriminant Analysis Principal Component (DAPC) methods. A total of 258 buffalo were sampled and genotyped. The results of admixture, multidimensional scaling (MDS), and DAPC showed a close relationship between the animals of different provinces. Two ecotypes indicated higher accuracy of 96% that the Area Under Curve (AUC) confirmed the obtained result of the SVM approach while the DAPC and RF approach demonstrated lower accuracy of 88% and 80 %, respectively. SVM method proved high accuracy compared with DAPC and RF methods and assigned animals to their herds with more accuracy. According to these results, buffaloes distributed in two different ecotypes are one breed, and therefore the same breeding program should be used in the future. The water buffalo ecotype of the northern provinces of Iran and Azerbaijan seem to belong to the same population. Keywords: Classification, Buffalo, Machine learning, SNP Chip data. |
Abstract : Light and Electron-Microscopic Studies on the Tubal Tonsil of the Buffalo (Bubalus bubalis)
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Abstract: The tubal tonsils of 12 adult buffaloes of the local mixed breed were studied using light and electron microscopy. The tonsillar mucosa lined by pseudostratified columnar ciliated epithelium with goblet cells was modified into lymphoepithelial, due to its association with underlying lymphoid tissue. The lymphoepithelial further modified into follicle-associated epithelium (FAE) characterised by absence of the ciliated cells, goblet cells and the presence of more lymphocytes. The FAE exhibited varying modifications and presented M-cells intimately associated with lymphocytes. At places, the change of the epithelium also showed the presence of specialised M-cell like cells without any association with lymphoid tissue. The lymphoid tissue was in the form of isolated lymphocytes, diffuse aggregations and follicles. The goblet cells of the respiratory epithelium and the glandular acinar cells showed positive activity for the different carbohydrate moieties like acidic and neutral mucopolysaccharides, glycogen, mucins, weakly sulfated acidic mucosubstances, hyaluronic acid and sialomucins. Scanning electron microscopy of the mucosal surface presented a dense mat of cilia, and the FAE exhibited a heterogeneous population of microvillus and M-cells. Transmission electron-microscopy demonstrated the different cell organelles of the various epithelia as well as the cellular profiles of the propria-submucosa, including the high endothelial venules where lymphocytes migration by both inter-endothelial and transvascular routes was also observed. The structural features of the tubal tonsil suggest that new strategies are required to explore this tonsil for targeted delivery of drugs and develop more effective vaccines by the intranasal route. Keywords: Buffalo, Follicle-associated epithelium, Lymphoepithelium, M-cells, Tubal tonsil. |
