Effect of Metal Ions, Solvents and Surfactants on the Activity of Protease from Aspergillus niger KIBGE-IB36
Pages 491-495

Hafsa Sattar, Afsheen Aman and Shah Ali Ul Qader
DOI: https://doi.org/10.6000/1927-5129.2017.13.80

Published: 13 September 2017

Abstract: Metal ions greatly impact on the enzymatic activity, they may form strong interaction by forming coordinate bond with enzyme-substrate at the catalytic site which may activate, inhibit or stabilized the enzyme molecules. In this study, extracellular protease from Aspergillus niger KIBGE-IB36 was precipitated with 40% ammonium sulfate. It was revealed that K⁺, Ba²⁺, Na⁺, Mg²⁺ Zn²⁺, Ca²⁺ boosted the protease activity whereas, Cs⁺, Mn²⁺, Cu²⁺, Ni²⁺, V²⁺, Co²⁺, Hg²⁺ and Al³⁺ showed to be inhibitor of protease. Dimethyl sulfoxide (5.0 mM) and methanol (5.0 mM) showed catalytic activity while ethanol at same concentration exhibited inhibitory effect. Protease activity augmented with Tween 80, while SDS, Triton X-100, EDTA and PMSF exhibited inhibitory effect.

Keywords: Metals, Inhibition, Activation, Protease, Aspergillus niger, Organic Solvents.

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