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Haematological Profiles of Lactating Nili-Ravi Buffaloes Under Heat Stress-Alleviated Conditions During Sub-tropical Summer Season
Pages 70-75
K.S. Das, J.K. Singh and V. Nayan

DOI: http://dx.doi.org/10.6000/1927-520X.2014.03.03.1

Published: 14 November 2014

 


Abstract: The experiment was conducted using forty two Nili-Ravi lactating buffaloes of Central Institute for Research on Buffaloes, Regional Station-Bir Dosanjh, Nabha, Patiala, Punjab to investigate the effect of heat stress-alleviated conditions on haematological profile in Nili-Ravi buffaloes during hot-dry (HD) and hot-humid (HH) season. All buffaloes were uniformly divided into two groups of twenty one in each group considering their lactation number, stage of lactation, body weight, dam’s milk yield and milk yield in current lactation. Out of which ten buffaloes from each group were selected for blood sampling considering those above factors. The control (T0) group were kept in a separate shed without any extra nutrient supplementation and modification in microclimate and management. The treatment (T1) group was supplemented with niacin@6 gm/buffalo/day, yeast@10 gm/buffalo/day and mustard oil @150gms/buffalo/day. They were provided curtains and mist fans in the shed. Feeding time, frequency and type of ration were also altered. During HD period, T0 group exhibited higher (P<0.002, P<0.05) WBC (17.2 x 109 /L), and lymphocyte (12.52 x 109 /L) counts and lymphocyte per cent (69.46 %) than T1 group (12.32 x 10 9 /L, 7.32 x 10 9 /L and 59.68%, respectively). Similar results were recorded during hot humid season in T0 and T1 group. The neutrophil per cent differed significantly between T0 and T1 group under HD (P<0.001) and HH (P<0.006) season. The study indicated that heat stress during sub-tropical summer months affected the haematology of lactating buffaloes and the stress could be reduced through the change (use of fans and curtains, nutritional supplementations, and feeding alterations together in the form of one package) of microclimate.

Keywords: Haematology, Heat stress-alleviation, Lactating buffalo.
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Journal of Buffalo Science

Prevalence of Serum Antibodies against Six Leptospira Serovars in Buffaloes in Tabriz, Northwestern Iran
Pages 76-81
Tolouei Mohammad

DOI: http://dx.doi.org/10.6000/1927-520X.2014.03.03.2

Published: 14 November 2014

 


Abstract: Leptospirosis is an important zoonotic infectious and its prevalence is unknown in buffalo in Iran particularly in Tabriz, northwest of the country. To survey the prevalence of Leptospira infection in buffaloes in Tabriz, blood samples were collected from 85 female buffaloes slaughtered in Tabriz industrial slaughterhouse from December 2008 to November 2009. Sera were stored at -20°C until they were examined. Sera were initially screened at serum dilution of 1:100 against six live antigens of Leptospira interrogans, Pomona, Canicola, Hardjo, Ballum, Icterohaemorrhagiae and Grippotyphosa by using the microscopic agglutination test (MAT).The samples were considered positive if 50% or more of agglutination of leptospire in a dilution tests serum of l:100 were observed. 30 serums (35.29%) at dilution 1:100 were positive against 1 or 2 of serovars. the highest prevalent serovar in buffalo was Grippotyphosa (51.3%), and fallowed whit Pomona (29.7%), canicola (10.8%) and Icterohaemorrhagiae (8.1%). All of the samples were seronegative for serovar Ballum and Hardjo. Statistical analysis of the results showed that the rates of the infection in the autumn-winter and spring–summer didn’t have significant difference (p > 0/05). The rate of the infection has been statistically increased with the aging (p<0/05) and the animals with 3 and 4 pair’s permanent teeth had the highest infection rates. The serological infection rate in buffaloes in Tabriz is relatively high and it appears that it is because of living type of buffaloes in water and swamp, thus consequently the preventive methods must be applied to control of the disease.

Keywords: Leptospirosis, seroprevalence, buffalo, MAT, Tabriz..
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Effective Environmental Factors on Milk Composition, Rennet Coagulation Time and Urea Content of in Anatolian Buffaloes Milk of Ilıkpınar Village Hatay Province
Pages 89-91
Özel Şekerden and Yahya Kemal Avşar

DOI: http://dx.doi.org/10.6000/1927-520X.2014.03.03.4

Published: 14 November 2014

 


Abstract: The objectives of this study were to investigate determining environmental factors on composition, renneting time, urea concentration, acidity, density and pH of Anatolian Buffaloes milk. As a total of 115 milk samples from 53 cows that were calved in the period of 2004 and 2005 years in 8 units of Ilıkpınar Village were collected in morning milkings in June, September, December and March. The cows were at their lactation days 30±15, 60±15, 90±15, 120±15, 150±15, 180±15, 210±15, 240±15 and 270±15. The milk samples were analysed for total dry matter, fat, protein, ash, density, pH, acidity, renneting time and urea content. Rennet coagulation time, urea, protein and fat contents were determined using Berridge, photometric, formal titration and Gerber methods, respectively. Data were classified as follows; lactation stages: 1 (30±15, 60±15, 90±15 days): 2 (120±15, 150±15, 180±15): 3 (210±15, 240±15, 270±15); calving year: 1 (2004), 2 (2005); calving season: 1 (January-May), 2 (September and October); month of samples collection: 1 (June), 2 (September), 3 (December), 4 (March); lactation order: 1 and 2 : 1, 3 and 4: 2, 5 and 6: 3. The effects of environmental factors of each variable were investigated separately and analysed using analysis of variance. Production mount in all the characteristics; calving year and lactation stage in most of the characteristics; lactation order on fat and protein contents; unit and calving season in some of the characteristics were found to be effective significantly. SPSS program was used in the statistical procedures.

Keywords: Buffalo, milk properties, variation sources.
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Biochemical and Fatty Acids Composition of Water Buffalo (Bubalus Bubalis) Follicular Fluid
Pages 82-88
J. Vencato, T. Badon, S. Bedin, P. Cogo, M. Simonato and C. Stelletta

DOI: http://dx.doi.org/10.6000/1927-520X.2014.03.03.3

Published: 14 November 2014

 


Abstract: Aim of this study was to characterize the biochemical and fatty acids composition of follicular fluid collected from follicles of different sizes and in different phases of ovarian cycle in water buffalo farmed in Italy. Ovaries were collected at slaughterhouse during the breeding season; follicular fluid was aspirated dividing samples in small and large follicles (< 6 mm and > 6 mm respectively) and in luteal and follicular phase. Biochemical analysis and gas-chromatography were performed. Biochemical and fatty acids composition were greatly influenced by both follicular dimension and phase of ovarian cycle. Biochemical composition and its variations were in agreement with previously study conducted in buffalo and other species. This is the first report of the fatty acids composition of buffalo follicular fluid. Twenty-two fatty acids were identified in follicular fluid; nine were saturated fatty acids, six monounsatured fatty acids and seven polyunsatured fatty acids. The most dominant fatty acids were linoleic acid, oleic acid, palmitic acid, stearic acid and arachidonic acid.All the identified fatty acids concentrations vary at least because of follicle dimension or phase, with the exception of γ-linoleic acid and arachidonic acid which concentrations remain stable in all classes.

Keywords: Water buffalo, follicular fluid, biochemestry, gas-chromatography, fatty acid.
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Growth Traits of Anatolian and Anatolian x Italian Crossbred Buffalo Calves Under the Village Conditions
Pages 92-96
Özel Şekerden

DOI: http://dx.doi.org/10.6000/1927-520X.2014.03.03.5

Published: 14 November 2014

 


Abstract: The study was carried out to compare growth performances of Anatolian and crossbred (Anatolian x Italian) buffalo calves (53 F1, 66 Anatolian, 26 F1xAnatolian) raised at Ilıkpınar Village of Kırıkhan District of Hatay Province. Body measurements were determined in the period of 0-12 months of age. The effects of genotype, sex, birth year effects on each characteristic in each age were investigated using GLM variance analysis. The means of each characteristic in each age for each genotype were calculated. Duncan test was used in comparison of the averages of each characteristic SPSS program was used in the statistical procedures. Genotype created significant variation in live weight at 1 and 6 months of ages respectively and on almost every body measurement almost in every age. Genotype* birth weight, genotype*sex, and genotype*birth year* sex interactions were found statistically significant in the point of view of various characteristics in various ages. Factors had significant effects on various characteristics in various ages. It can be said that, F1 growth was the most speedly and Anatolian buffaloes stayed behind of the other genotypes from the point of view of all the traits.

Keywords: Buffalo, Anatolian, Italian, Body measurements.
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