In Vitro Evaluation of the Antiinflammatory and Anticancer Activities of Compounds from the Plants Casearia sylvestris and Zanthoxylum monophyllum
Pages 114-121
Omar A. Dupuy L., José A. Bonilla V., Renato Murillo M., Peter Taylor, María J. Abad, Lorena I. González P., Olmedo Otero and Johanna Juliao A.

DOI: http://dx.doi.org/10.6000/1929-2279.2015.04.03.2

Published: 13 August 2015

Abstract: The anthocyanins, coumarins, and casearins are interesting compounds due to their chemical and biological properties. In the present study, the in vitro effect of 4´-O-methyl-gallocatechin (an anthocyanin), columbianatin (a coumarin), and casearin G on the mitogenic response and phagocytic activity from whole blood cells, was evaluated. In addition, the cytotoxic effect of these compounds on the cell lines B16/BL6 (murine melanoma) and COS-7 (kidney fibroblasts transfected with SV40 virus) was measured by a colorimetric assay (MTS/PMS, IC₅₀: inhibitory concentration) and the sulphorhodamine B assay (GI₅₀: growth inhibition, TGI: total growth inhibition, LC₅₀: lethal concentration). The results show that 4´-O-methyl-gallocatechin and columbianatin reduced lymphoproliferation. Columbianatin reduced both the phagocytic index and the percentage of phagocytic monocytes/macrophages. Casearin G showed both cytotoxic (IC₅₀, LC₅₀) and cytostatic (GI₅₀) effects against the tumor cells, B16/BL6 (IC₅₀ = 30.8 μM; GI₅₀ = 12.4 μM; LC₅₀ = 34.7 μM) and COS-7 (IC₅₀ = 137.3 μM; GI₅₀ = 3.8 μM; LC₅₀ = 29.9 μM). In conclusion, 4´-O-methyl-gallocatechin and columbianatin showed immunosuppressive properties in vitro while casearin G was the most cytotoxic.

Keywords: 4´-O-methyl-gallocatechin, columbianatin, casearin G, peripheral blood mononuclear cells, B16/BL6 melanoma, COS-7.