Novel Isolates of Lactobacilli from Crop of Algerian Poultry as Potential Probiotic for Food Industry
Pages 82-90
Tayeb Idoui and Mohamed Sifour

DOI: http://dx.doi.org/10.6000/1927-3037.2016.05.03.3

Published: 04 October 2016

Abstract: This study was aimed at selecting novel strains of Lactobacillus from crop of Algerian poultry. One hundred forty (140) lactobacilli strains were isolated and examined for their potentiality probiotic properties. From these isolated strains, nine appear to possess a probiotic value and highlighted a noticeable heterogeneity. The isolate L. plantarum G₁ showed the best inhibitory activity against several indicator strains. Furthermore, the results showed that culture and neutralized supernatants exhibited varying degrees of inhibitory activity against strains of enterobacteria from poultry origin. The tested strains were acid resistant and were also bile tolerant. Antibiotic resistance, co-aggregation activity and hydrophobicity percentage were strain-dependent. Moreover, six strains were able to adhere to epithelial cells. Finally, six Lactobacillus strains, such as strain L. plantarum G_1, L. plantarum PC₂, L. viridesencs G₃, L. helveticus PC₆, L. delbrueckii ssp delbrueckii G₇ and L. fermentum PC₈, showed essential probiotic properties. The identity of the best strain L. plantarum G1 was confirmed by 16S rRNA gene sequence using PCR.