Platelets Mitochondrial Function Depends on Coenzyme Q₁₀ Concentration in Human Young, Not in Elderly Subjects - Pages 67-76
Anna Gvozdjáková, Zuzana Sumbalová, Jarmila Kucharská, Anežka Chládeková, Zuzana Rausová, Oľga Vančová, Mária Kubalová, Zuzana Kuzmiaková, Michal Nemec, Oľga Uličná and Viliam Mojto

DOI: https://doi.org/10.6000/1929-5634.2018.07.03.1

Published: 16 August 2018

Abstract: Ageing is characterized by a progressive decline in the physiological functions of various organs. Mitochondrial alterations occurring in senescence. Antioxidants, including coenzyme Q₁₀ concentration, fall with ageing and contribute to enhanced oxidative stress age-related diseases. The impairment of platelet mitochondrial function occurs in a broad spectrum of diseases.

The aim of this study was to evaluate mitochondrial function in platelets in elderly and young human controls and correlate it with a concentration of coenzyme Q₁₀. Platelets mitochondrial function was determined by the use of High-Resolution Respirometry method.

We did not find significantly decreased platelet mitochondrial function in elderly subjects. Dependence of platelets mitochondrial respiratory chain function and ATP production at Complex I on a concentration of coenzyme Q₁₀ in platelets and whole blood in young not in elderly human volunteers was documented. This dependence was not found for Complex II in any group. Platelet mitochondrial coenzyme Q₁₀ concentration was insufficient for improving platelet mitochondrial function in elderly human subjects. Recommending supplementation with coenzyme Q₁₀ in elderly and aged humans is waranted.

High-Resolution Respirometry method offers a perspective to diagnose mitochondrial energy metabolism which might be useful for further studies in patients with mitochondrial disorders. Our results could contribute to the explanation of platelets mitochondrial function in elderly and aged human subjects.

Keywords: Platelets, mitochondria, High-Resolution Respirometry, coenzyme Q_10, age.

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