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Abstract : Forensic Identification of Suspected Poached Wild Boar (Sus scrofa affinis)
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Abstract: A case of suspected wild boar death due to dynamite blast by poachers was received by us for confirmation. The skin sample was collected and examined histologically for identification based on hair follicle arrangement in the horizontal section of skin. The horizontal sections of skin from both domestic and wild boar was stained in order to study the distribution of hair follicles. Linear arrangement of hair follicles comprising three primary follicles in a row was observed in both domestic and suspected wild boar. Based on circumstantial evidence it was concluded that it may be belonging to wild boar. Keywords: Forensic identification, wild boar, poaching, histology, hair follicle arrangement.Download Full Article |
Abstract : Pesticides and Veterinary Drugs Residues in Conventional Meat: A Food Safety Issue
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Abstract: In the current scenario the most of people are well aware with health issues. Food safety is generally related with the quality of food i.e. whether the food product is standardised as according to national or international norms set by the statutory organisations. People can compromise with the nutritive values of food but not with their safety aspects. The meat and meat products carry the burden of harmful agents according to the production methods. Now-a-days the feedlot animals are being reared either through the natural farming (organic farming) or conventional farming method. Those methods produce safe and healthier meat because there is no use of harmful chemical agents’ viz., pesticides, herbicides, hormones, growth promoters, veterinary drugs and etc. On the other hand, in the conventional farming, all these chemical agents are used to enhance animal growth. Several chemical agents like pesticides and veterinary drugs residues may cause harmful health implications viz., teratogenicity, carcinogenicity, hypersensitivity reactions, gut bacterial resistance, toxicity and many more health problems in human beings. It is the thrust of today to replace the conventional meat with the organic meat to check the use of harmful chemical agents for a healthy social life. Keywords: Meat, organic farming, conventional farming, food safety.Download Full Article |
Abstract : Standardization of a SYBR Green Based Real-Time PCR System for Detection and Molecular Quantification of Babesia bovis and B. bigemina in Water Buffaloes (Bubalus bubalis)
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Abstract: Water buffalo (Bubalus bubalis) is a potential reservoir for Babesia bovis and B. bigemina in tropical regions, but the epidemiological evidence of their reservoir competence is limited, especially due to the lack of diagnostic tests capable of detecting and quantifying the low-level parasitemia present in the carrier animals. In this paper we present the standardization process of a SYBR Green based real-time PCR system (qPCR), consisting of two single qPCR assays, for the detection and quantification of B. bovis and/or B. bigemina. Both assays were optimized in similar protocols, including reagent concentrations and thermocycling parameters, so it is possible its use as a multiple qPCR in a single run. Both single assays showed a suitable analytical performance, especially by allowing detection of a greater number of carrier animals when compared with nested PCR assays (nPCR) against a reference panel of 60 DNA samples extracted from blood of both, infected- and non-infected buffaloes. Furthermore, a mathematical algorithm to convert the qPCR outcomes in percent of infected red blood cell was used, and was found that the estimated parasitemia in carrier buffaloes within the reference sample panels were close to those described in carrier cattle. This method could be a useful tool for epidemiological studies on the participation of the bubaline specie in the epidemic process of bovine babesiosis. Keywords: Babesia bovis, B. bigemina, water buffalo, parasitemia, qPCR, standardization.Download Full Article |
Abstract : Selection of Biomarkers from Differentially Expressed Genes in Leukocytes of Buffalos Treated with Recombinant Bovine Somatotropin: The Importance of Sample Size for Reliable Discriminating Systems
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Abstract: The research on biomarkers to detect livestock treated with recombinant bovine somatotropin (rbST) is still an open issue. In fact, beyond undertaking confirmation methods, there is the need to develop simple and inexpensive screening tests. In this direction, some proposals have been forwarded, mostly involving the measurement of circulating molecules, whereas the possibility of using biomarkers related to gene expression is a field under investigation. The present study was carried out on sixteen buffalos, eight of which treated with rbST. Blood samples were collected six times during the treatment to investigate on the presence of differentially expressed genes in leukocytes. Analysis with the microarray technique was performed on two sampling moments, in order to obtain a first selection of genes. Further analysis was carried out by real time RT-PCR, in order to create a discriminating linear system. A study on the variation of the error related to the number of samples included in statistics was also performed. Results showed that, including an increasing number of samples to build the discriminating algorithm, the b-error grows and tends to stabilize on 6.5%. This study clearly shows the paramount importance of including a proper number of samples to obtain reliable algorithms.. Keywords: Recombinant bovine somatotropin, Buffalos, Biomarkers, Screening tests, Sample size.Download Full Article |
