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Biochemical and Fatty Acids Composition of Water Buffalo (Bubalus Bubalis) Follicular Fluid
Pages 82-88
J. Vencato, T. Badon, S. Bedin, P. Cogo, M. Simonato and C. Stelletta

DOI: http://dx.doi.org/10.6000/1927-520X.2014.03.03.3

Published: 14 November 2014

 


Abstract: Aim of this study was to characterize the biochemical and fatty acids composition of follicular fluid collected from follicles of different sizes and in different phases of ovarian cycle in water buffalo farmed in Italy. Ovaries were collected at slaughterhouse during the breeding season; follicular fluid was aspirated dividing samples in small and large follicles (< 6 mm and > 6 mm respectively) and in luteal and follicular phase. Biochemical analysis and gas-chromatography were performed. Biochemical and fatty acids composition were greatly influenced by both follicular dimension and phase of ovarian cycle. Biochemical composition and its variations were in agreement with previously study conducted in buffalo and other species. This is the first report of the fatty acids composition of buffalo follicular fluid. Twenty-two fatty acids were identified in follicular fluid; nine were saturated fatty acids, six monounsatured fatty acids and seven polyunsatured fatty acids. The most dominant fatty acids were linoleic acid, oleic acid, palmitic acid, stearic acid and arachidonic acid.All the identified fatty acids concentrations vary at least because of follicle dimension or phase, with the exception of γ-linoleic acid and arachidonic acid which concentrations remain stable in all classes.

Keywords: Water buffalo, follicular fluid, biochemestry, gas-chromatography, fatty acid.
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Effective Environmental Factors on Milk Composition, Rennet Coagulation Time and Urea Content of in Anatolian Buffaloes Milk of Ilıkpınar Village Hatay Province
Pages 89-91
Özel Şekerden and Yahya Kemal Avşar

DOI: http://dx.doi.org/10.6000/1927-520X.2014.03.03.4

Published: 14 November 2014

 


Abstract: The objectives of this study were to investigate determining environmental factors on composition, renneting time, urea concentration, acidity, density and pH of Anatolian Buffaloes milk. As a total of 115 milk samples from 53 cows that were calved in the period of 2004 and 2005 years in 8 units of Ilıkpınar Village were collected in morning milkings in June, September, December and March. The cows were at their lactation days 30±15, 60±15, 90±15, 120±15, 150±15, 180±15, 210±15, 240±15 and 270±15. The milk samples were analysed for total dry matter, fat, protein, ash, density, pH, acidity, renneting time and urea content. Rennet coagulation time, urea, protein and fat contents were determined using Berridge, photometric, formal titration and Gerber methods, respectively. Data were classified as follows; lactation stages: 1 (30±15, 60±15, 90±15 days): 2 (120±15, 150±15, 180±15): 3 (210±15, 240±15, 270±15); calving year: 1 (2004), 2 (2005); calving season: 1 (January-May), 2 (September and October); month of samples collection: 1 (June), 2 (September), 3 (December), 4 (March); lactation order: 1 and 2 : 1, 3 and 4: 2, 5 and 6: 3. The effects of environmental factors of each variable were investigated separately and analysed using analysis of variance. Production mount in all the characteristics; calving year and lactation stage in most of the characteristics; lactation order on fat and protein contents; unit and calving season in some of the characteristics were found to be effective significantly. SPSS program was used in the statistical procedures.

Keywords: Buffalo, milk properties, variation sources.
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Growth Traits of Anatolian and Anatolian x Italian Crossbred Buffalo Calves Under the Village Conditions
Pages 92-96
Özel Şekerden

DOI: http://dx.doi.org/10.6000/1927-520X.2014.03.03.5

Published: 14 November 2014

 


Abstract: The study was carried out to compare growth performances of Anatolian and crossbred (Anatolian x Italian) buffalo calves (53 F1, 66 Anatolian, 26 F1xAnatolian) raised at Ilıkpınar Village of Kırıkhan District of Hatay Province. Body measurements were determined in the period of 0-12 months of age. The effects of genotype, sex, birth year effects on each characteristic in each age were investigated using GLM variance analysis. The means of each characteristic in each age for each genotype were calculated. Duncan test was used in comparison of the averages of each characteristic SPSS program was used in the statistical procedures. Genotype created significant variation in live weight at 1 and 6 months of ages respectively and on almost every body measurement almost in every age. Genotype* birth weight, genotype*sex, and genotype*birth year* sex interactions were found statistically significant in the point of view of various characteristics in various ages. Factors had significant effects on various characteristics in various ages. It can be said that, F1 growth was the most speedly and Anatolian buffaloes stayed behind of the other genotypes from the point of view of all the traits.

Keywords: Buffalo, Anatolian, Italian, Body measurements.
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Changes of Milk Production and Reproductive Performance of Dairy Buffaloes in Nepal During Recent 15 Years
Pages 34-37
Y. Hayashi, M.K. Shah and H. Kumagai

DOI: http://dx.doi.org/10.6000/1927-520X.2014.03.02.1

Published: 18 June 2014

 


Abstract: Consumption of buffalo milk has been increasing in Nepal. Thus, further development of dairy buffalo is required for efficient production. However, the changes of milk production and reproductive performance of buffaloes in the country remain obscure. Hence, the study is conducted for identifying the dairy production and reproduction of buffaloes raised in Nepal during 15 years. The records of daily milk yield (MY) from July 1997 to July 2012 were collected at Livestock Development Farm, Nepal. Lactation MY, 305 days MY, lactation length, MY per day of lactation, peak MY, days to attain peak MY, calving interval and dry period were calculated. The results were summarized every 5 years (Period 1: July 1997 – July 2002, Period 2: July 2002 – July 2007, Period 3: July 2007 – July 2012). The lactation MY, 305 days MY, daily MY per lactation and peak MY were lower in Period 1 than in Period 2 and 3. On the other hand, the days to attain peak MY was longer in Period 1 than in the other periods. However, the lactation length had no significant difference among the periods. The milk production was assumed to improve from Period 1 to Period 2 and 3. The calving interval was longer in Period 3 than in Period 2. The dry period was longest in Period 3. The efficiency of buffalo reproduction during the recent 5 years assumed to be declined. The deterioration of reproduction possibly has been caused by changes in nutritional and environmental conditions during the surveyed period.

Keywords: Buffalo, change, milk production, Nepal, reproduction.

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