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Abstract: The paper deals with some topics of important aspects of food safety and application of principles of food physics in the sector of agriculture, food technology, biotechnology, including questions of well balanced nutrition, as well. One of the most important and widely applied field of biotechnology is the food technology. Food production and processing of quality food and safe food are today of primary importance. Food production is based on the principles of GAP, GMP and GHP. Recently the industrial food processing is focused dominantly on the quality, and one of the basic requirements of the quality is the safety. There are various methods and techniques to produce safe food. The modern food technologies and quality measurements (quality control, quality assurance) involve the application of different physical methods – e.g. high pressure, pulsing electrical field, nondestructive techniques (e.g. NMR, NIR-NIT, PAS, INAA) for chemical composition determination, radiation techniques, nanofiltration and reverse osmosis (RO) – as well. Using e.g. ionizing radiation (nuclear methods) and non-ionizing radiation technologies it is possible to fulfil a lot of important expectations: decrease of microbial contamination, improve of sensory properties, increase of storability, etc. The paper deals with questions of up-to-date, with sport motion combined diet, helping in keeping the health, as well. In the last decades a lot of information were distributed concerning several viewpoints of bodyweight reduction and optimation of bodymaa. How should we know that it is useful and not unhealthy? A suitable Nutrition software (AOPNEI, Analysing and Optimation Program for Nourishment and Energy Intake) – developed at the Department of Food Chemistry and Nutrition of the Faculty of Food Science, Corvinus University – can help. Keywords: Bodymass, food processing, food safety, physical methods, quality assurance, nutrition, radiation methods. |
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Abstract: In this study, two Lactobacillus (LAB) strains namely, Lactobacillus rhamnosus EMCC 1105 (L. rhamnosus) and Lactobacillus gasseri EMCC 1930 (L. gasseri) were tested for their antagonistic activities against Pseudomonas aeruginosa (P. aeruginosa), Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) which are known to be frequently implicated in biofilm infections. The acidic cell free culture supernatant (CFS) of 24 h and 48 h cultures of both LAB stains showed antimicrobial effects against the three pathogens in radial diffusion assay. These effects were abolished upon neutralization of CFS indicating that this effect was due to acids only. Both LAB strains could effectively inhibit the biofilm formation of the three test pathogens and largely replaced them on polystyrene surfaces as demonstrated by crystal violet staining, viable count and scanning electron microscopy. Both of the tested LAB strains could inhibit the protease productivity of S. aureus in 24 h and 48 h dual species-biofilms. The supernatant of 24 h-dual biofilms of P. aeruginosa with L. gasseri also showed a significantly lower protease activity compared to that of P. aeruginosa individual biofilm. Neither LAB strains affected phospholipase C production by the test pathogens when they co-exist during biofilm formation. The different preparations of LAB strains caused no significant change in the levels of gamma interferon expressed by peripheral blood mononuclear cells in response to stimulation by the test pathogens in vitro. In conclusion, L. gasseri and L. rhamnosus can be considered as promising tools for combating biofilm infections. Keywords: Probiotics, Lactobacillus rhamnosus, Lactobacillus gasseri, Biofilm, Antibiofilm, Gamma interferon. |
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Abstract: Wine is an agricultural product with very high commerce price variation, which is highly affected by quality ratings. Therefore, quality rating is particularly important for both industry and consumers. However, absence of clear concepts on what constitutes wine quality makes the perception of quality highly subjective, and it is usual for tasters to disagree on the quality rating of a specific wine. For this purpose, a Feedforward Neural Network (FNN) could be trained in order to predict wine quality. In this study, a new FNN method was developed to predict the accurate wine quality based on major sensory characteristics as FNN inputs, and to improve the ability of a taster, groups of tasters, or consumers, to rate wine by taking into account previous decisions. Specifically, five principle sensory characteristics of wines were used as inputs (Aging in Barrel, Aroma Intensity, Body, Astringency, and Acidity) in a rating range 1-3. As outputs, the quality ratings of wines in a range 70-100 were considered. The FNN was created in MATLAB with 1 hidden layer, 5 neurons and 1 output layer. For ratings divided in 5 categories the accuracy was 53% with the use of the FNN, as opposed to the accuracy of 36% achieved by Multiple Linear Regression. For ratings divided in 9 categories the accuracy was 90%. This method may allow each individual or group of tasters to introduce their own data to produce a more objective rating by taking into account previous decisions (subjective) that have accumulated in the database. Keywords: Wine rating, sensory evaluation, Feedforward Neural Networks, regression analysis. |
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Abstract: The antimicrobial capability of oil containing medium-chain fatty acids and their corresponding monoglycerides, known as Enhanced Virgin Coconut Oil (EVCO) against microorganisms isolated from bovine mastitis milk was studied. EVCO contains an effective amount of medium-chain free fatty acids (caprylic, capric and lauric acid) and their corresponding derivatives (monocaprylin, monocaprin and monolaurin) can act as antimicrobial agent with broad spectrum of antimicrobial properties. An in vitro assessment of EVCO against bovine mastitis microorganisms isolated from locally collected mastitis milk samples was conducted to determine its minimum bactericidal concentration (MBC>99). In general, EVCO exhibited its great antimicrobial capability to kill all mastitis microorganisms isolated from the mastitis milk sample at different concentrations of MBC>99 value. In fact, EVCO was found more effective to kill Mycoplasma sp. and gram positive microbes rather than gram negative microbes, especially in Mycoplasma bovis, Acholeplasma oculi, Enterococcus sp., Streptococcus sp. and Staphylococcus aureus with lower MBC>99 values in comparison with the synthetic antibiotic, Mastivet. A further investigation of the antimicrobial activities of EVCO against Staphylococcus aureus and Mycoplasma bovis against time revealed that EVCO was more efficient in killing these two microorganisms in a shorter time at a lower concentration as opposed to Mastivet. These findings indicated that EVCO has great potential application in postmilking teat germicide, which can be used as a preventive measure to control mastitis cases, especially against environmental mastitis pathogens. Keywords: Mastitis, Enhanced Virgin Coconut Oil (EVCO), Mastivet, Antimicrobial Activity. |
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Abstract: Phytochemical screening (saponins, tannins, steroids, alkaloids, flavonoids, phenols and glycosides) of four medicinal plant seeds (Jatropha curcas, Simmondsia chinensis (Jojoba), Moringa oleifera and Datura metel) extracted by aqueous, ethanol and Folch solvents, were examined for their antimicrobial activity against three types of plant pathogenic fungi namely; Botrytis cinerea, Fusarium oxysporum and Rhizoctonia solani, in addition to four types of bacteria, namely; Bacillus cereus, Staphylococcus aureus, Ralstonia solanacearum and Pesudomonas aeruginosa using disc diffusion paper. Results revealed that different concentrations of aqueous extracts were more effective against bacterial activity compared to fungal activity, except for D. metel aqueous extract which showed no antifungal effect and very weak effect on only two of the tested bacteria. B. cereus was more sensitive to J. curcas aqueous extract, while P. aeruginosa was more sensitive to S. chinensis and M. oleifera aqueous extracts. On the other hand, results showed that J. curcas and M. oleifera ethanol extracts were more effective on Staph. aureus growth, while S. chinensis and D. metel did not have any effect on any of the fungi or bacteria under study. The evaluation of the antifungal and antibacterial effect did not confirm the broad spectrum of S. chinensis Folch extract, while M. oleifera and D. metel were more effective on reducing R. solani growth. Also F. oxysporum was affected by J. curcas Folch extract only at high concentrations. These findings support that the traditional use of the plant extracts in the treatment of different infections caused by pathogenic microbes is valuable and should be taken in consideration. Keywords: Phytochemical screening, antimicrobial activities, Jatropha curcas, Simmondsia chinensis (Jojoba), Moringa oleifera, Datura metel. |