jpans
|
|
Abstract: The gastrointestinal microoganisms encompass thousands of bacterial species that constitute a relatively stable ecosystem inside human body. Dysbiosis is an imbalance condition of beneficiary microbacteria cause by newer microorganism. This research aimed to investigate the effect of L. plantarum IS-10506 as probiotics supplementation on total bacterial and Enterobacteriaceae count; and also to identify the dysbiosis causing bacteria. Total bacterial and Enterobacteriaceae number on both control and test group were determined by Total Plate Count method. Identification of dysbiosis related bacteria were determined by fermentation test, gram staining, and Microbact™ 12A kit. The TPC result of total bacterial population on control group was 222.67 x 106 cfu/ml, while the probiotics induced group was 210.33 x 106 cfu/ml. As for the TPC result for Enterobacteriaceae population from control group was 12.00 x 106 cfu/ml, Enterobacteriaceae population in the probiotics induced group was 11.66 x 106 cfu/ml. Four genera related to dysbiosis has been isolated from the intestinal sample and all are rod-shaped Gram negative bacteria. In conclusion, the use of L. plantarum IS-10506 as food supplementation has reduced the total bacterial count. Four genera of dysbiosis related bacteria has been identified from the intestinal sample which include Eschericia, Serratia, Enterobacter, and Citrobacter. Keywords: Dysbiosis, Probiotics, Identification, Total Plate Count, Rabbit Intestine. |
|
|
Abstract: This study examines the influence of the tissue preparation "NICAVET 2500" on an organism of mammals with use of rodent models of acute inflammation. It is established that action of a preparation leads to decrease in ESR. Hematologic and biochemical parameters also testify to anti-inflammatory action of "NICAVET 2500". Results of histological and morphometric research of a spleen and a thymus show processes of proliferation and migration of immunocytes, testifying to activization of immune reactions. In a thymus of rats of experimental group in comparison with control an increase in the dimensions of thymic lobules and also an increase in quantity of Hassal's bodies, testifying to intensification of synthesis of the thymic hormones participating in process of an immunopoesis are revealed. As a result of the use of the preparation an increase of ability of an organism to resist alteration and also essential decrease in a level of development of inflammatory reaction of an organism are observed. The conducted research demonstrates the expressed immunomodulatory action of a preparation "NICAVET 2500" at an experimental model of inflammation. |
|
|
Abstract: Organic Cation Transporter 1 (OCT1) is the primary transporter of metformin mainly located in hepatocytes, which plays an important role in metformin action to inhibit gluconeogenesis so as to reduce blood glucose. Genetic polymorphism of SLC22A1 that encodes OCT1, one of which is rs622342, has been widely reported and proven to decrease the antidiabetic effect of metformin. This study aimed to design primers and to obtain an optimum condition for polymerase chain reaction (PCR) process that can detect the genetic polymorphism of SLC22A1 rs622342. Primers were computationally designed in primer 3 webpage and analyzed with Primer BLAST and Oligo Analyzer. Optimization of PCR condition was conducted for temperatures of denaturation, annealing, and elongation as well as for the number of cycles in PCR process. Sensitivity test was performed on PCR condition using a variety of volumes and DNA template qualities undergoing multiple freeze-thaw cycles. The obtained pair, forward primer (5’- CAG AGA GAA TCA GTG AGC TGT G-3’) and reverse primer (5’- CCC AGG CTG GTC TTT TTA AG-3’), was proven to be capable of amplifying DNA sequence containing SNPs in rs622342 at 96°C denaturation, 60°C annealing, and 72°C elongation temperatures with a 30-cycle iteration. Such PCR condition could amplify DNA with 0.2 µL of template volume and 7 freeze-thaw cycles. Therefore, in addition to the selected primer pairs and PCR condition to analyze SNPs in rs622342, this study also recommends that the volume of DNA template having undergone multiple freeze-thawing be increased if the amplicon PCR products are unqualified. Keywords: Metformin, PCR, Primer design, OCT1, rs622342. |
|
|
Abstract: The aim was to investigate prooxidant-antioxidant system in the blood and brain homogenates functional activity in rats with acute hypoxia model with different deuterium/protium (D/H) ratios in drinking diet. Studies have shown that consuming DDW (–665 ‰) within 8 weeks lead to deuterium concentration decrease in blood plasma at 317 ‰ and brain at 209 ‰ of rats, in comparison to control group, consuming natural water. DDW consumption before hypoxia modeling in rats improves antioxidant defense enzymes (catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase) activity in the blood, increasing its antioxidant potential by 20 %, while free radical oxidation intensity in plasma and biomolecules peroxide modification rate in erythrocytes. Also, in brain tissues consuming DDW, there were no abnormalities in catalase, superoxide dismutase activity, and it was noted increase (by 71 %) in reduced thiol-containing compounds concentration, which reduces nerve hypoxiс cell damage risk. Neuroprotective effect presence is confirmed by higher (by 32 %) antioxidant activity markers of lyophilized brain tissues, and by free radical oxidation lower intensity (by 13 %) and biomolecules oxidative modification rate (by 16 %) in these lyophilized tissues. Thus, the advisability of using DDW neuroprotective effects in cerebral circulation disturbance in experimental and clinical practice. Keywords: ddw, hypoxia, brain, catalase, SOD. |
|
|
Abstract: This study was to investigate the antioxidant activity of three different extracts of propolis (Ethanolic 50%, methanolic 100% and aqueous extracts) with some of their physicochemical aspects. Ethanolic extract was analyzed by LC-MS, while the methanolic extract was analyzed by GC-MS in order to screen polyphenols and volatile compounds in the sample, the identity of these components were achieved by means of comparing their mass spectra with previous published studies. Forrier transformation infrared spectroscopy (FTIR) was conducted on the methanolic extract and crude propolis samples to gain an overview of some physical characteristics. The antioxidant activities of the three extracts were measured by DPPH scavenging ability and FRAP assay. LC-MS and GC-MS allowed the identification of 19 phenolic and 17 volatile compounds. The most abundant phenolics found in our sample correspond with p-Coumaric benzyl ester, Pinobanksin-3-O-acetate, Caffeic acid benzyl ester and pinocembrin, as they showed the highest peaks in the total ion chromatogram (TIC). In addition, the most abundant volatile compounds showed a high level of agreement with δ-Cadinene and γ- Terpinen.Furthermore, Ethanol 50% extract showed the strongest antioxidant activity, which has a significant correlation with its high polyphenolic content. Keywords: Propolis, antioxidant, Liquid Chromatography-Mass Spectrometry, Gas Chromatography-Mass Spectrometry, Folin-Ciocalteu. |