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Abstract:The experiment was conducted on effects of bio-pesticides on biology of Chrysoperla carnea F. under laboratory conditions. The neem and datura leaf extracts were used as bio-pesticides and their effect was compared with confidor at 26±2°C, 65±5% R. H and photoperiod (16L: 8D) in the Department of Entomology, Sindh Agriculture University, Tandojam, Pakistan during 2014. The results shows that the incubation periods of eggs of C. carnea feeding on Aphis gossypii treated with neem, datura and confidor was 2.2, 2.5 and 3.6 days respectively. The result indicated that the total larval developmental period was 17.03, 13.3 and 15.09, respectively. The pupal period of C. carnea was 8.82 on neem, 10.9 on datura and 12.33 days on confidor. The result further revealed that the pre oviposition period of C. carnea was 6.35 on neem, 5.5 on datura and 3.6 on confidor. The oviposition period was 34.42 on neem, 30.6 on datura and 26.4 on confidor. The post oviposition period was significantly different was 8.5 days on neem 6.9 on datura and 4.7 on confidor. The maximum fecundity per female of C. carnea was 448.38 days on neem, 435.67 on datura and 413.67 on confidor. Similarly, maximum egg hatching percentage of C.carnea was recorded on neem followed by datura and confidor. However, the maximum egg mortality (37.65%) was recorded on confidor. However, minimum mortality of 1st, 2nd and 3rd instar larvae was recorded due to neem leaf extracts followed by datura and confidor. The pupal mortality was seen more on neem followed by datura and confidor. The highest adult mortality was obtained on neem followed by datura and confidor insecticide. Keywords: Chrysoperla carnea F., Bio-pesticides, Biology, Synthetic pesticides. Download Full Article |
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Abstract: Cities all around the world being crowded rapidly with accelerating urban growth and resultant urbanization particularly in developing countries. Currently, Bahawalpur City is also experiencing rapid urban growth and change in its urban and municipal limits. Therefore, the key objectives of this research were to overview the urban population growth with passage of time, expansion in urban area and resultant delimitations in municipal limits of the city. Data about population figures and spatial change have been derived from various sources including census reports, Tehsil Municipal Administration (TMA), Punjab Development statistics etc. Periodical urban growth of the city is divided into four phases characterized with notable events that geared up the urban growth; particularly the last phase has brought more rapid changes. The population of the city is also surging rapidly. Urban area of Bahawalpur City was extended from 4,308.40 acres in 1974 to 7,373.9 acres in 2010, and had caused substantial reduction in agricultural land area and significant addition in residential area. Resultantly, City’s municipal limits also have been altered and reclassified due to this urban stress and extended enormously with considerable fluctuations in agricultural and non-agricultural land area. Presently, City is continuously expanding outward, mostly in the form of housing colonies, roads, shops etc. Keywords: Urban growth, municipal limits, agricultural land area, Bahawalpur |
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Abstract: Various studies have reported that global warming causes unstable climate and serious impact on physical environment and public health. The increasing incidence of dengue case is now a priority health issue and has become a health burden for Pakistan. In this study it has been investigated that spatial pattern of environment causes the emergence or increasing rate of dengue fever incidence that effects the population and its health. The climatic or environmental and the Dengue Fever (DF) case data was processed by coding, editing, tabulating, recoding and restructuring and finally applying different statistical methods, techniques and procedures for the analysis and interpretation. Five climatic variables which we have studied are precipitation (P), Maximum temperature (Mx), Minimum temperature (Mn), Humidity (H) and Wind speed (W) collected from 1980-2012. The data on Dengue Fever cases in Karachi for the period 2010 to 2012 are available and reported on weekly basis. Principal Component 1 (PC1) for all groups of the period can be interpreted as the General atmospheric condition. PC2 the second important climate factor for dengue period (2010-2012) comes out contrast between precipitation and wind speed. PC3 is the weighted difference between maximum temperature and wind speed. PC4 is the contrast between maximum and wind speed. Negative Binomial and Poisson regression model are used to correlate the dengue fever incidence to climatic variable and principal component (PC) score. Due to the problems of over dispersion the Poisson models are not useful for interpretation through Negative Binomial model we found that relative humidity causes an increase on the chances of dengue occurrence by 1.71% times. While maximum temperature positively influence on the chances dengue occurrence by 19.48% times. Minimum temperature affects on the chances of dengue occurrence by 11.51% times. Wind speed is effecting negatively on the weekly occurrence of dengue fever by 7.41%times. Keywords: Principal component analysis, Dengue Fever, Negative Binomial Regression model, Poisson Regression model. |
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Abstract: In search of new potential therapeutic agents, 8-Hydroxyquinoline which is a highly active antifungal and antibacterial agent was subjected to Mannich reaction. Different combinations of aromatic aldehyde and aromatic amines were used for Mannich reaction on 8-Hydroxyquinoline from which one new 7-[α-(anilino)-3',4'-dimethoxybenzyl]-8-hydroxyquinoline (3) and two known Mannich bases (1,2) were obtained. Keywords: 8-Hydroxyquinoline, Oxine, Mannich Base, Mannich Reaction. |
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Abstract: Objective:To acquire monoclonal hybridoma cell lines against fummonisin B1(FB1) and extract monoclonal antibody against FB1. Methods: Coupling antigens of FB1-KLH and FB1-BSA with chemical methods and immune 6-8 weeks old female BALB/c mice with FB1-KLH. Integrating spleen cells with sp2/0 myeloma cells to acquire hybridoma cell lines secreting McAb against FB1. The method of multiple subclones was used to select cell lines stably secreting McAb. McAbs was got from ascites and purified. The subclass of antibody was measured and the molecular weight was identified. The specificity and sensitivity of McAb were identified with indirect competitive inhibition ELISA. Results:The results of serum from immuned mice showed that after five times of immunization the titer stables at 1×10-6, and the McAb belongs to IgG1 subclass, the light chain was κ, the molecular weight of heavy and light chain were 55kDa and 32kDa, respectively. ELISA results showed that McAb could react with FB1. The linear range indirect competitive inhibition ELISA is 10-500ng/ml. Conclusion:The monoclonal hybridoma cell lines and the high specificity,high sensitivity of FB1-McAb was Keywords: Fummonisin B1, hybridomacell lines, monoclonal antibody. Download Full Article |